2026-05-02T06:54:54Zhttps://repository.ucc.edu.co/server/oai/request

oai:repository.ucc.edu.co:20.500.12494/497092024-08-20T21:15:38Zcom_20.500.12494_36578com_20.500.12494_36577col_20.500.12494_41230

Hernández López, Juan Carlos Latz, E. Urcuqui Inchima, S. 2023-05-24T16:21:39Z 2023-05-24T16:21:39Z 01/01/2013 https://doi.org/10.1159/000353902 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84889820913&doi=10.1159%2f000353902&partnerID=40&md5=c3cb5bba2776cb898287c152a9a55a59 03005526 https://hdl.handle.net/20.500.12494/49709 Hernandez Lopez Juan carlos,Latz E.,Urcuqui-Inchima S..HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages.Intervirology. 2013. 57. (1):p. 36-42 Background/Aims: Inflammasomes are multimolecular complexes that regulate caspase-1. They act as sensors for endogenous and exogenous signals, and mediate the processing of pro-IL-1ß into its secreted, biologically active form. The NLRP3 inflammasome and IL-1ß are particularly interesting because they are required for efficient control of viral infections. Indeed, HIV-1 induces expression of NLRP3 and IL-1ß in healthy controls, but not in HIV-1-infected patients. Here we evaluate whether HIV-1 can induce activation of the NLRP3 inflammasome. Methods: Human primary monocyte-derived macrophages were infected with HIV-1 in the absence or presence of classical NLRP3 inflammasome activators, and IL-1ß release was assessed by ELISA. Results: HIV-1 initiates the priming signal for NLRP3 inflammasome activation through the NF-?B-associated pathway in human primary monocyte-derived macrophages. Furthermore, priming of NLRP3 activation in response to HIV-1 was independent of the viral envelope, since similar results were observed with HIV-1 and pseudotyped HIV-1 lacking the env gene. Conclusion: Our findings suggest that HIV-1 infection promotes IL-1ß secretion by inducing the first signal for NLRP3 inflammasome activation, a phenomenon that may contribute to AIDS progression. Copyright © 2013 S. Karger AG, Basel. 0000-0002-9200-5698 juanc.hernandezl@campusucc.edu.co 36-42 S. Karger AG ARTICLE CARRIER PROTEINS CELLS, CULTURED CONTROLLED STUDY CRYOPYRIN CYTOKINE RELEASE ENVELOPE GENE ENZYME LINKED IMMUNOSORBENT ASSAY ENZYME-LINKED IMMUNOSORBENT ASSAY HIV-1 HUMAN HUMAN CELL HUMAN IMMUNODEFICIENCY VIRUS 1 HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION HUMANS IMMUNOGLOBULIN ENHANCER BINDING PROTEIN INFLAMMASOME INFLAMMASOMES INTERLEUKIN 1BETA INTERLEUKIN-1BETA MACROPHAGE MACROPHAGES MONOCYTE NONHUMAN PRIORITY JOURNAL PROTEIN INDUCTION SIGNAL TRANSDUCTION VIRUS ENVELOPE HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages Artículo http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_970fb48d4fbd8a85 info:eu-repo/semantics/article http://purl.org/redcol/resource_type/ART info:eu-repo/semantics/publishedVersion Intervirology info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 Publicationmetadata.only