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oai:repository.ucc.edu.co:20.500.12494/418052024-08-20T21:21:13Zcom_20.500.12494_36578com_20.500.12494_36577col_20.500.12494_41230

Hernández López, Juan Carlos Latz E. Urcuqui-Inchima S. 2021-12-16T22:15:48Z 2021-12-16T22:15:48Z 2013 https://doi.org/10.16925/di.v18i23.1292 03005526 https://hdl.handle.net/20.500.12494/41805 Hernandez JC,Latz E,Urcuqui S. HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages. Intervirology. 2013. 57. (1):p. 36-42. . Background/Aims: Inflammasomes are multimolecular complexes that regulate caspase-1. They act as sensors for endogenous and exogenous signals, and mediate the processing of pro-IL-1ß into its secreted, biologically active form. The NLRP3 inflammasome and IL-1ß are particularly interesting because they are required for efficient control of viral infections. Indeed, HIV-1 induces expression of NLRP3 and IL-1ß in healthy controls, but not in HIV-1-infected patients. Here we evaluate whether HIV-1 can induce activation of the NLRP3 inflammasome. Methods: Human primary monocyte-derived macrophages were infected with HIV-1 in the absence or presence of classical NLRP3 inflammasome activators, and IL-1ß release was assessed by ELISA. Results: HIV-1 initiates the priming signal for NLRP3 inflammasome activation through the NF-?B-associated pathway in human primary monocyte-derived macrophages. Furthermore, priming of NLRP3 activation in response to HIV-1 was independent of the viral envelope, since similar results were observed with HIV-1 and pseudotyped HIV-1 lacking the env gene. Conclusion: Our findings suggest that HIV-1 infection promotes IL-1ß secretion by inducing the first signal for NLRP3 inflammasome activation, a phenomenon that may contribute to AIDS progression. Copyright © 2013 S. Karger AG, Basel. 0000-0002-9200-5698 juanc.hernandezl@campusucc.edu.co 42-36 S. Karger AG cryopyrin immunoglobulin enhancer binding protein inflammasome interleukin 1beta immunoglobulin enhancer binding protein interleukin 1beta article controlled study cytokine release enzyme linked immunosorbent assay human human cell Human immunodeficiency virus 1 infection macrophage priority journal protein induction signal transduction virus envelope Article envelope gene Human immunodeficiency virus 1 Human immunodeficiency virus 1 infection macrophage monocyte nonhuman Carrier Proteins Cells Cultured Enzyme-Linked Immunosorbent Assay HIV-1 Humans Inflammasomes Interleukin-1beta Macrophages Human immunodeficiency virus 1 HIV-1 induces the first signal to activate the NLRP3 inflammasome in monocyte-derived macrophages Artículo http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_970fb48d4fbd8a85 info:eu-repo/semantics/article http://purl.org/redcol/resource_type/ART info:eu-repo/semantics/publishedVersion Intervirology info:eu-repo/semantics/closedAccess http://purl.org/coar/access_right/c_14cb Publicationmetadata.only