2026-04-30T13:51:42Zhttps://repository.ucc.edu.co/server/oai/request

oai:repository.ucc.edu.co:20.500.12494/417262024-08-20T21:18:17Zcom_20.500.12494_36578com_20.500.12494_36577col_20.500.12494_41230

Hernández López, Juan Carlos Stevenson M. Latz E. Urcuqui-Inchima S. 2021-12-16T22:15:44Z 2021-12-16T22:15:44Z 2012 https://doi.org/10.15446/anpol.v31n94.78239 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053407586&doi=10.7203%2fCIRIEC-E.93.10327&partnerID=40&md5=bf4e34a3b306679b0594288956a8839c 08892229 https://hdl.handle.net/20.500.12494/41726 Hernández JC,Stevenson M,Latz E,Urcuqui S. HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro. AIDS Res Hum Retroviruses. 2012. 28. (10):p. 1313-1328. . Toll-like receptors (TLRs) play a critical role in innate immunity against pathogens. Their stimulation induces the activation of NF-?B, an important inducer of HIV-1 replication. In recent years, an increasing number of studies using several cells types from HIV-infected patients indicate that TLRs play a key role in regulating the expression of proinflammatory cytokines and viral pathogenesis. In the present study, the effect of HIV-1 stimulation of monocyte-derived macrophage (MDM) and peripheral blood mononuclear cell (PBMC) subpopulations from healthy donors on the expression and functions of TLR2 and TLR4 was examined. In addition, and to complete the in vitro study, the expression pattern of TLR2 and TLR4 in 49 HIV-1-infected patients, classified according to viral load and the use of HAART, was determined and compared with 25 healthy subjects. An increase of TLR expression and production of proinflammatory cytokines were observed in MDMs and PBMCs infected with HIV-1 in vitro and in response to TLR stimulation, compared to the mock. In addition, an association between TLR expression and up-regulation of CD80 in plasmacytoid dendritic cells (pDCs) was observed. The ex vivo analysis indicated increased expression of TLR2 and TLR4 in myeloid dendritic cells (mDCs), but only of TLR2 in monocytes obtained from HIV-1-infected patients, compared to healthy subjects. Remarkably, the expression was higher in cells from patients who do not use HAART. In monocytes, there was a positive correlation between both TLRs and viral load, but not CD4+ T cell numbers. Together, our in vitro and ex vivo results suggest that TLR expression and function can be up-regulated in response to HIV-1 infection and could affect the inflammatory response. We propose that modulation of TLRs represents a mechanism to promote HIV-1 replication or AIDS progression in HIV-1-infected patients. © 2012, Mary Ann Liebert, Inc. 0000-0002-9200-5698 juanc.hernandezl@campusucc.edu.co 1328-1313 Mary Ann Liebert Inc. abacavir amprenavir B7 antigen beta actin CD123 antigen CD14 antigen CD4 antigen cytokine didanosine efavirenz fosamprenavir immunoglobulin enhancer binding protein interleukin 1beta interleukin 6 lamivudine lopinavir messenger RNA nelfinavir nevirapine saquinavir stavudine toll like receptor 2 toll like receptor 4 tumor necrosis factor alpha zidovudine acquired immune deficiency syndrome adolescent adult article CD4+ T lymphocyte cell subpopulation clinical article controlled study cytokine production ex vivo study female gene expression regulation highly active antiretroviral therapy human human cell Human immunodeficiency virus 1 Human immunodeficiency virus 1 infection Human immunodeficiency virus infected patient immune response immunomodulation in vitro study in vivo study inflammation innate immunity macrophage male monocyte myeloid dendritic cell nonhuman peripheral blood mononuclear cell plasmacytoid dendritic cell priori HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro Artículo http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_970fb48d4fbd8a85 info:eu-repo/semantics/article http://purl.org/redcol/resource_type/ART info:eu-repo/semantics/publishedVersion AIDS RES HUM RETROV info:eu-repo/semantics/closedAccess http://purl.org/coar/access_right/c_14cb Publicationmetadata.only