Publicación:
Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis

dc.contributor.authorNavarro-Quiroz E.
dc.contributor.authorPacheco-Lugo L.
dc.contributor.authorNavarro Quiroz, Roberto Carlos
dc.contributor.authorLorenzi H.
dc.contributor.authorEspaña-Puccini P.
dc.contributor.authorDíaz-Olmos Y.
dc.contributor.authorAlmendrales L.
dc.contributor.authorOlave V.
dc.contributor.authorGonzalez-Torres H.
dc.contributor.authorDiaz-Perez A.
dc.contributor.authorDominguez A.
dc.contributor.authorIglesias A.
dc.contributor.authorGarcía R.
dc.contributor.authorAroca-Martinez G.
dc.date.accessioned2021-12-16T22:15:53Z
dc.date.available2021-12-16T22:15:53Z
dc.date.issued2017
dc.description.abstractRenal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It’s estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals. The aim of this study was to evaluate the relative abundance of circulating microRNAs in peripheral blood from Colombian patients with LN-IV. Circulating miRNAs in plasma of patients with diagnosis of LN-IV were compared with individuals without renal involvement (LNN group) and healthy individuals (CTL group). Total RNA was extracted from 10 ml of venous blood and subsequently sequenced using Illumina. The sequences were processed and these were analyzed using miRBase and Ensembl databases. Differential gene expression analysis was carried out with edgeR and functional analysis were done with DIANA-miRPath. Analysis was carried out using as variables of selection fold change (2 o -2) and false discovery rate (0.05). We identified 24 circulating microRNAs with differential abundance between LN-IV and CTL groups, fourteen of these microRNAs are described for the first time to lupus nephritis (hsa-miR-589-3p, hsa-miR-1260b, hsa-miR-4511, hsa-miR-485-5p, hsa-miR-584-5p, hsa-miR-543, hsa-miR-153-3p, hsa-miR-6087, hsa-miR-3942-5p, hsa-miR-7977, hsa-miR-323b-3p, hsa-miR-4732-3p and hsa-miR-6741-3p). These changes in the abundance of miRNAs could be interpreted as alterations in the miRNAs-mRNA regulatory network in the pathogenesis of LN, preceding the clinical onset of the disease. The findings thus contribute to understanding the disease process and are likely to pave the way towards identifying disease biomarkers for early diagnosis of LN. © 2017 Navarro-Quiroz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es
dc.description.emailroberto.navarroq@ucc.edu.coes
dc.format.extent13-1es
dc.identifierhttps://doi.org/10.17533/udea.rfo.v28n2a5
dc.identifierhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85021626638&doi=10.3303%2fCET1757296&partnerID=40&md5=c05c5ffed767411ff5ef0dbedf8921bb
dc.identifier.bibliographicCitationNavarro E,Pacheco L,Navarro R,Lorenzi H,España P,Díaz Y,Almendrales L,Olave V,Gonzalez H,Diaz A,Dominguez A,Iglesias A,García R,Aroca G. Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis. PLoS One. 2017. 12. (11):p. 1-13. .es
dc.identifier.issn19326203es
dc.identifier.urihttps://hdl.handle.net/20.500.12494/41911
dc.publisherPublic Library of Sciencees
dc.relation.ispartofjournalPlos Onees
dc.rights.accessrightsinfo:eu-repo/semantics/closedAccesses
dc.subjectmicroRNAes
dc.subjectmicroRNA 1260bes
dc.subjectmicroRNA 153 3pes
dc.subjectmicroRNA 323b 3pes
dc.subjectmicroRNA 3942 5pes
dc.subjectmicroRNA 4511es
dc.subjectmicroRNA 4732 3pes
dc.subjectmicroRNA 485 5pes
dc.subjectmicroRNA 543es
dc.subjectmicroRNA 584 5pes
dc.subjectmicroRNA 589 3pes
dc.subjectmicroRNA 6087es
dc.subjectmicroRNA 6741 3pes
dc.subjectmicroRNA 7977es
dc.subjectunclassified druges
dc.subjectadolescentes
dc.subjectadultes
dc.subjectagedes
dc.subjectArticlees
dc.subjectblood samplinges
dc.subjectclinical articlees
dc.subjectcohort analysises
dc.subjectColombianes
dc.subjectcomparative studyes
dc.subjectcontrolled studyes
dc.subjectdata basees
dc.subjectdisease classificationes
dc.subjectDNA fingerprintinges
dc.subjectfemalees
dc.subjectgene controles
dc.subjectgene expressiones
dc.subjectgene identificationes
dc.subjectgene mutationes
dc.subjecthumanes
dc.subjectlupus erythematosus nephritises
dc.subjectmalees
dc.subjectonset agees
dc.subjectpathogenesises
dc.subjectRNA analysises
dc.subjectRNA extractiones
dc.subjectRNA sequencees
dc.titleProfiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritises
dc.typeArtículo
dspace.entity.typePublication

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