Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

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dc.creatorCarvalho A.
dc.creatorPelaez Vargas, Alejandro
dc.creatorHansford D.J.
dc.creatorFernandes M.H.
dc.creatorMonteiro F.J.
dc.creator.mailalejandro.pelaezv@campusucc.edu.coes
dc.date.accessioned2021-12-16T22:15:41Z
dc.date.available2021-12-16T22:15:41Z
dc.date.issued2016
dc.description.abstractA primary goal in bone tissue engineering is the design of implants that induce controlled, guided, and rapid healing. The events that normally lead to the integration of an implant into bone and determine the performance of the device occur mainly at the tissue-implant interface. Topographical surface modification of a biomaterial might be an efficient tool for inducing stem cell osteogenic differentiation and replace the use of biochemical stimuli. The main goal of this work was to develop micropatterned bioactive silica thin films to induce the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) only through topographical stimuli. Line and pillar micropatterns were developed by a combination of sol-gel/soft lithography and characterized by scanning electron microscopy, atomic force microscopy, and contact angle measurements. hMSCs were cultured onto the microfabricated thin films and flat control for up to 21 days under basal conditions. The micropatterned groups induced levels of osteogenic differentiation and expression of osteoblast-associated markers higher than those of the flat controls. Via comparison of the micropatterns, the pillars caused a stronger response of the osteogenic differentiation of hMSCs with a higher level of expression of osteoblast-associated markers, ALP activity, and extracellular matrix mineralization after the cells had been cultured for 21 days. These findings suggest that specific microtopographic cues can direct hMSCs toward osteogenic differentiation. © 2016 American Chemical Society.es
dc.description.orcid0000-0001-7582-2760es
dc.format.extent1100-1091es
dc.identifierhttps://doi.org/10.12804/revsalud13.03.2015.12
dc.identifierhttps://dialnet.unirioja.es/servlet/articulo?codigo=6499267
dc.identifier.bibliographicCitationCarvalho A,Pelaez A,Hansford DJ,Fernandes MH,Monteiro FJ. Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells. Langmuir. 2016. 32. (4):p. 1091-1100. .es
dc.identifier.issn07437463es
dc.identifier.urihttp://hdl.handle.net/20.500.12494/41655
dc.publisherAmerican Chemical Societyes
dc.relation.ispartofLangmuires
dc.rights.accessRightsDesconocidoes
dc.subjectAtomic force microscopyes
dc.subjectBonees
dc.subjectCell culturees
dc.subjectCellses
dc.subjectCytologyes
dc.subjectScanning electron microscopyes
dc.subjectSol-gelses
dc.subjectStem cellses
dc.subjectSurface treatmentes
dc.subjectTissuees
dc.subjectTissue engineeringes
dc.subjectBasal conditionses
dc.subjectBiochemical stimulies
dc.subjectBone tissue engineeringes
dc.subjectExtracellular matriceses
dc.subjectHuman bone marrow derived mesenchymal stem cellses
dc.subjectOsteogenic differentiationes
dc.subjectSilica thin filmses
dc.subjectTissue implantses
dc.subjectThin filmses
dc.subjectsilicon dioxidees
dc.subjectcell culturees
dc.subjectcell differentiationes
dc.subjectchemistryes
dc.subjectcytologyes
dc.subjecthumanes
dc.subjectmesenchymal stroma celles
dc.subjectmicrotechnologyes
dc.subjectosteoclastes
dc.subjectreverse transcription polymerase chain reactiones
dc.subjecttissue engineeringes
dc.subjectCell Differentiationes
dc.subjectCellses
dc.subjectCulturedes
dc.subjectHumanses
dc.subjectMesenchymal Stromal Cellses
dc.subjectMicrotechnologyes
dc.subjectOsteoclastses
dc.subjectReverse Transcriptase Polymerase Chain Reactiones
dc.subjectSilicon Dioxidees
dc.subjectTissue Engineeringes
dc.titleEffects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cellses
dc.typeArtículo
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