Proteomic profile of human monocytic cells infected with dengue virus

Martínez Gutiérrez, Marlén; Martínez Betancur, Viviana

Publicación:
Proteomic profile of human monocytic cells infected with dengue virus

dc.contributor.author	Martínez Gutiérrez, Marlén
dc.contributor.author	Martínez Betancur, Viviana
dc.date.accessioned	2017-08-24T14:20:16Z
dc.date.available	2017-08-24T14:20:16Z
dc.date.issued	2016-11
dc.description	Artículo proteómica dengue	es
dc.description.abstract	Objective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein quantity. Results In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially (five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed (two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 kDa protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 kDa protein AA1, tubulin beta, enolase 1, pyruvate kinase, transaldolase and phospholipase C-alpha. Conclusions Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection.	es
dc.description.email	marlen.martinezg@campusucc.edu.co	es
dc.identifier.bibliographicCitation	Martinez Gutierrez, M., & Martínez Betancur. (2016). Proteomic profile of human monocytic cells infected with dengue virus. Asian Pac J Trop Biomed 2016; 6(11): 914–923. https://doi.org/10.1016/j.apjtb.2016.01.004	es
dc.identifier.uri	https://hdl.handle.net/20.500.12494/1350
dc.publisher	Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina Veterinaría y Zootecnia, Bucaramanga, Colombia, 00000	es
dc.publisher.place	Bucaramanga	es
dc.publisher.program	Medicina veterinaria y zootecnia	es
dc.relation.isversionof	http://www.sciencedirect.com/science/article/pii/S2221169115307917	es
dc.rights.accessrights	info:eu-repo/semantics/openAccess	es
dc.rights.cc	Licencia CC
dc.subject	Virus	es
dc.subject	Dengue	es
dc.subject	Macrophages	es
dc.subject	Proteomic	es
dc.subject	Monocytes	es
dc.subject	U937 cells	es
dc.title	Proteomic profile of human monocytic cells infected with dengue virus	es
dc.type	Artículo
dspace.entity.type	Publication