Publicación:
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi ganoderma sp. and lentinus sp.

dc.contributor.authorFlórez-Sampedro L.
dc.contributor.authorZapata Builes, Wildeman
dc.contributor.authorOrozco L.P.
dc.contributor.authorMejía A.I.
dc.contributor.authorArboleda C.
dc.contributor.authorRugeles M.T.
dc.date.accessioned2021-12-16T22:15:56Z
dc.date.available2021-12-16T22:15:56Z
dc.date.issued2016
dc.description.abstractBackground: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Objetives: In this study we evaluated the anti-HIV-1 activity of the enzymatic extracts (EE) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EE and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for green fluorescent protein –GFP– and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The EEs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EE from Lentinus sp. had the best selectivity index (SI: 8.3). Conclusions: These results suggest the potential anti-HIV-1 activity of the EE for the exploration of an alternative therapy against HIV-1 infection. © 2016, Universidad de Antioquia. All rights reserved.es
dc.description.emailwildeman.zapatab@campusucc.edu.coes
dc.description.orcid0000-0002-7351-8738es
dc.format.extent118-109es
dc.identifierhttps://doi.org/10.18632/oncoscience.393
dc.identifier.bibliographicCitationFlórez L,Zapata W,Orozco LP,Mejía AI,Arboleda C,Rugeles MT. In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi ganoderma sp. and lentinus sp. Vitae. 2016. 23. (2):p. 109-118. .es
dc.identifier.issn21452660es
dc.identifier.urihttps://hdl.handle.net/20.500.12494/41978
dc.publisherUniversidad de Antioquiaes
dc.relation.ispartofjournalRevista VITAEes
dc.rights.accessrightsinfo:eu-repo/semantics/closedAccesses
dc.subjectantivirus agentes
dc.subjectfungal extractes
dc.subjectgreen fluorescent proteines
dc.subjecthexadimethrine bromidees
dc.subjectlaccasees
dc.subjectRNA directed DNA polymerasees
dc.subjectantiviral activityes
dc.subjectapoptosises
dc.subjectArticlees
dc.subjectcell proliferationes
dc.subjectcontrolled studyes
dc.subjectcytotoxicity assayes
dc.subjectenzyme linked immunosorbent assayes
dc.subjectflow cytometryes
dc.subjectGanodermaes
dc.subjectHuman immunodeficiency virus 1es
dc.subjectLentinuses
dc.subjectlinear regression analysises
dc.subjectMTT assayes
dc.subjectnonhumanes
dc.subjectpolymerase chain reactiones
dc.subjectreverse transcriptiones
dc.subjectultraviolet spectrophotometryes
dc.subjectvirus recombinantes
dc.subjectvirus replicationes
dc.titleIn vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi ganoderma sp. and lentinus sp.es
dc.typeArtículo
dspace.entity.typePublication

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