In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.

Flórez Sampedro, Laura; Zapata Builes, Wildeman; Orozco, Lina P.; Mejía, Amanda I.; Arboleda Echavarría, Carolina; Rugeles, María T.

Publicación:
In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.

dc.contributor.author	Flórez Sampedro, Laura
dc.contributor.author	Zapata Builes, Wildeman
dc.contributor.author	Orozco, Lina P.
dc.contributor.author	Mejía, Amanda I.
dc.contributor.author	Arboleda Echavarría, Carolina
dc.contributor.author	Rugeles, María T.
dc.date.accessioned	2017-08-23T20:52:51Z
dc.date.available	2017-08-23T20:52:51Z
dc.date.issued	2016
dc.description	Artículo Científico	es
dc.description.abstract	Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Objetives: In this study we evaluated the anti-HIV-1 activity of the enzymatic extracts (EE) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EE and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for green fluorescent protein –GFP– and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The EEs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EE from Lentinus sp. had the best selectivity index (SI: 8.3). Conclusions: These results suggest the potential anti-HIV-1 activity of the EE for the exploration of an alternative therapy against HIV-1 infection.	es
dc.description.email	wildeman.zapatab@campusucc.edu.co	es
dc.description.tableofcontents	Abstract. -- Materials and methods. -- Results. -- Discussion. -- Reference.	es
dc.identifier.bibliographicCitation	Flórez Sampedro, L.; Zapata, W; Orozco, L. P.; Mejía, A. I.; Arboleda, C.; Rugeles, M. T. (2016). In vitro Anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the Fungi Ganoderma sp. and Lentinus sp. Vitae. Vitae, Revista de la Facultad de Ciencias Farmacéuticas y Alimentarias. 23(2), pp. 109-118.	es
dc.identifier.uri	https://hdl.handle.net/20.500.12494/1319
dc.publisher	Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000	es
dc.publisher.place	Medellín	es
dc.publisher.program	Medicina	es
dc.relation.isversionof	http://aprendeenlinea.udea.edu.co/revistas/index.php/vitae/article/view/23524/20782958	es
dc.rights.accessrights	info:eu-repo/semantics/openAccess	es
dc.rights.cc	Licencia CC
dc.subject	Human immunodeficiency virus type 1 (HIV-1)	es
dc.subject	Ganoderma	es
dc.subject	Lentinus laccase	es
dc.subject	Antiviral activity	es
dc.subject	Natural products	es
dc.title	In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.	es
dc.type	Artículo
dspace.entity.type	Publication