Please use this identifier to cite or link to this item: https://repository.ucc.edu.co/handle/20.500.12494/46183
Title: An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses
Author: Alvarez-Diaz, Diego A.
Valencia-Álvarez, Emmanuel
Rivera, Jorge A.
Rengifo, Aura C.
Usme-Ciro, Jose A.
Pelaez-Carvajal, Dioselina
Lozano-Jimenez, Yenny Y.
Torres-Fernandez, Orlando
Email autor: jose.usmec@campusucc.edu.co
metadata.dc.description.cvlac: https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000318507
Issue Date: 9-Jun-2021
Advisor / Validator: Usme-Ciro, Jose A.
Keywords: Arbovirus
Carga viral
RT-qPCR
Chikungunya
Dengue
Zika
Abstract: The real-time reverse transcription-polymerase chain reaction (real-time RT-qPCR) has become a leading technique for the detection and quantification of arboviruses, including Chikungunya, Dengue, and Zika viruses. In this study, an updated real-time RT-qPCR assay was designed and evaluated together with a synthetic positive-control chimeric RNA for the simultaneous detection and quantification of Chikungunya, Dengue, and Zika viruses. Amplification assays were performed to verify the construct integrity and optimal reaction/thermal cycling conditions. The analytical sensitivity of the assay was determined for each virus in single and multiplex reactions, as well as the performance in the detection and viral load quantification of experimental samples. The real-time RT-qPCR assay presented here allowed for the simultaneous detection and quantification of Chikungunya, Dengue, and Zika viruses and could be applied in several studies where the accurate quantification of viral genomes is required.
Program: Medicina
Headquarters: Santa Marta
Publisher: Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina, Santa Marta
metadata.dc.type: Artículos Científicos
Citation: Álvarez-Díaz DA, Valencia-Álvarez E, Rivera JA, Rengifo AC, Usme-Ciro JA, et al. (2021). An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses. Infect Genet Evol. 2021 Jun 9;93:104967. doi: 10.1016/j.meegid.2021.104967.https://repository.ucc.edu.co/handle/20.500.12494/46183
Resource reference: https://www.sciencedirect.com/science/article/abs/pii/S1567134821002641?via%3Dihub
Appears in Collections:Medicina



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