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Title: HIV replication is associated to inflammasomes activation, IL-1ß, IL-18 and caspase-1 expression in GALT and peripheral blood
Author: Feria M.G.
Taborda N.A.
Hernandez Lopez, Juan Carlos
Rugeles M.T.
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Issue Date: 2018
Keywords: apoptosis regulatory protein
calcium binding protein
caspase recruitment domain signaling protein
interleukin 18
interleukin 18 protein
interleukin 1beta
interleukin 1beta converting enzyme
NLRC4 protein
NLRP1 protein
PYCARD protein
signal transducing adaptor protein
CD4+ T lymphocyte
host pathogen interaction
Human immunodeficiency virus infection
lymphoid tissue
mononuclear cell
virus load
virus replication
Adaptor Proteins
Signal Transducing
Apoptosis Regulatory Proteins
Calcium-Binding Proteins
CARD Signaling Adaptor Proteins
Caspase 1
CD4-Positive T-Lymphocytes
HIV Infections
Host-Pathogen Interactions
Lymphoid Tissue
Viral Load
Virus Replication
Abstract: Background Human immunodeficiency virus (HIV) promotes an inflammatory process, leading to the progressive loss of the functional capacity of the immune system. The HIV infection induces alterations in several tissues, but mainly in the gut-associated lymphoid tissue (GALT). However, the degree of GALT deterioration varies among infected individuals. In fact, it has been shown that HIV-controllers, who spontaneously control viral replication, exhibit a lower inflammatory response, and a relative normal frequency and function of most of the immune cells. Inflammasomes are molecular complexes involved in the inflammatory response, being NLRP1, NLRP3, NLRC4, AIM2 and Pyrin inflammasomes, the best characterized so far. These complexes regulate the maturation of cytokines of the IL-1 family, including IL-1ß and IL-18. These cytokines have been associated with immune activation and expansion of HIV target cells, promoting viral replication. Interesting, some reports indicate that HIV induces the activation of the NLRP3 inflammasome, but the role of this, and other inflammasomes during HIV infection, especially in GALT, remains unclear. Objective To compare the relative expression of inflammasome components and the proinflammatory response related to their activity, between HIV-progressors and HIV-controllers. Methods GALT biopsies and peripheral blood mononuclear cells (PBMCs) from 15 HIV-controllers and 15 HIV-progressors were obtained. The relative expression of the following inflammasome components were evaluated by RT-PCR: NLRP3, NLRC4, NLRP1, AIM2, ASC, Cas-pase-1, IL-1ß and IL-18. In addition, plasma concentration of IL-18 was evaluated as an indicator of baseline proinflammatory status. Finally, in supernatants of PBMCs in vitro stimulated with inflammasome agonists, the concentrations of IL-1ß and IL-18 were quantified by ELISA. © 2018 Feria et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publisher: Public Library of Science
metadata.dc.type: Artículo
Citation: Feria MG,Taborda NA,Hernandez JC,Rugeles MT. HIV replication is associated to inflammasomes activation, IL-1ß, IL-18 and caspase-1 expression in GALT and peripheral blood. PLoS One. 2018. 13. (4):p. 1-14. .
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Appears in Collections:Artículos Científicos

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