Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12494/41889
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Title: High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals
Author: Aguilar-Jiménez W.
Zapata Builes, Wildeman
Caruz A.
Rugeles M.T.
Email autor: wildeman.zapatab@campusucc.edu.co
Issue Date: 2013
Keywords: beta defensin 2
beta defensin 3
interleukin 10
interleukin 1beta
messenger RNA
transforming growth factor beta
tumor necrosis factor alpha
vitamin D receptor
adult
article
case control study
controlled study
cross-sectional study
enzyme linked immunosorbent assay
exposure
female
gene expression
genital mucosa
human
Human immunodeficiency virus 1 infection
human tissue
major clinical study
male
mouth mucosa
mucosa
peripheral blood mononuclear cell
reverse transcription polymerase chain reaction
upregulation
vitamin blood level
Adult
beta-Defensins
Cross-Sectional Studies
Cytokines
Disease Resistance
Female
HIV Infections
HIV Seronegativity
HIV-1
Humans
Interleukin-10
Male
Mouth Mucosa
Mucous Membrane
Receptors
Calcitriol
RNA
Messenger
Transcription
Genetic
Vitamin D
Young Adult
Abstract: Vitamin D (VitD) is an endogenous immunomodulator that could protect from HIV-1 infection reducing immune activation and inducing the expression of anti-HIV-1 peptides. To establish a correlation between VitD and natural resistance to HIV-1 infection, a case-control study using blood and mucosa samples of 58 HIV-1-exposed but seronegative (HESN) individuals, 43 HIV-1 seropositives (SPs) and 59 non-exposed healthy controls (HCs) was carried out. The VitD concentration in plasma was determined by ELISA, and mRNA relative units (RU) of VDR, IL-10, TGF-ß, TNF-a and IL-1ß in peripheral blood mononuclear cells (PBMCs), oral and genital mucosa was quantified by qRT-PCR. mRNA levels of human beta-defensin (HBD) -2 and -3 were previously reported and used for correlations. Significantly higher levels of VitD were found in plasma as well as higher mRNA RU of VDR in PBMCs, and in genital mucosa from HESN compared to HCs. In addition, higher mRNA RU of TNF-a, IL-1ß and IL-10, and lower mRNA RU of TGF-ß were found in PBMC from HESNs compared to HCs. We also observed higher IL-10 mRNA RU in genital mucosa of HESNs compared to HCs, and the mRNA levels of TNF-a in oral and genital mucosa of SPs were higher compared to HESNs. Furthermore, positive correlations between VDR and IL-10 mRNA RU in PBMCs and genital mucosa of HESNs were found. Finally, HBD-2 and HBD-3 mRNA RU were positively correlated with VDR mRNA expression in oral mucosa from HESNs. These results suggest that high levels of VitD and its receptor are associated with natural resistance to HIV-1 infection. Up-regulation of the anti-inflammatory IL-10, and the induction of anti-HIV-1 defensins in mucosa might be part of the mechanisms involved in this association. However, further studies are required to define causal associations. Copyright: © 2013 Aguilar-Jiménez et al.
Type: Artículo
Citation: Aguilar W,Zapata W,Caruz A,Rugeles MT. High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals. PLoS One. 2013. 8. (12):e82717. .
Other Identifiers: https://doi.org/10.17533/udea.iatreia.v29n4a06
https://revistas.ucr.ac.cr/index.php/aie/article/view/28679
Appears in Collections:Artículos Científicos

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