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dc.creatorTorres S.-
dc.creatorHernandez Lopez, Juan Carlos-
dc.creatorGiraldo D.-
dc.creatorArboleda M.-
dc.creatorRojas M.-
dc.creatorSmit J.M.-
dc.creatorUrcuqui-Inchima S.-
dc.description.abstractBackground: Dengue hemorrhagic fever (DHF) is observed in individuals that have pre-existing heterotypic dengue antibodies and is associated with increased viral load and high levels of pro-inflammatory cytokines early in infection. Interestingly, a recent study showed that dengue virus infection in the presence of antibodies resulted in poor stimulation of Toll-like receptors (TLRs), thereby facilitating virus particle production, and also suggesting that TLRs may contribute to disease pathogenesis. Methodology/Principal Findings: We evaluated the expression levels of TLR2, 3, 4 and 9 and the co-stimulatory molecules CD80 and CD86 by flow cytometry. This was evaluated in monocytes, in myeloid and plasmacytoid dendritic cells (mDCs and pDCs) from 30 dengue patients with different clinical outcomes and in 20 healthy controls. Increased expression of TLR3 and TLR9 in DCs of patients with dengue fever (DF) early in infection was detected. In DCs from patients with severe manifestations, poor stimulation of TLR3 and TLR9 was observed. In addition, we found a lower expression of TLR2 in patients with DF compared to DHF. Expression levels of TLR4 were not affected. Furthermore, the expression of CD80 and CD86 was altered in mDCs and CD86 in pDCs of severe dengue cases. We show that interferon alpha production decreased in the presence of dengue virus after stimulation of PBMCs with the TLR9 agonist (CpG A). This suggests that the virus can affect the interferon response through this signaling pathway. Conclusions/Significance: These results show that during dengue disease progression, the expression profile of TLRs changes depending on the severity of the disease. Changes in TLRs expression could play a central role in DC activation, thereby influencing the innate immune response. © 2013 Torres et
dc.description.provenanceMade available in DSpace on 2021-12-16T22:15:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPLOS NEGLECT TROP Des
dc.subjectalpha interferones
dc.subjectB7 antigenes
dc.subjectCD123 antigenes
dc.subjectCD14 antigenes
dc.subjectCD86 antigenes
dc.subjectglycoprotein p 15095es
dc.subjectimmunoglobulin Ges
dc.subjectimmunoglobulin Mes
dc.subjecttoll like receptores
dc.subjecttoll like receptor 2es
dc.subjecttoll like receptor 3es
dc.subjecttoll like receptor 4es
dc.subjecttoll like receptor 9es
dc.subjectanimal celles
dc.subjectclinical articlees
dc.subjectcontrolled studyes
dc.subjectdendritic celles
dc.subjectDengue viruses
dc.subjectdisease coursees
dc.subjectdisease severityes
dc.subjectenzyme linked immunosorbent assayes
dc.subjectflow cytometryes
dc.subjecthuman celles
dc.subjectimmune responsees
dc.subjectInfluenza virus Aes
dc.subjectperipheral blood mononuclear celles
dc.subjectprotein expressiones
dc.subjectreverse transcription polymerase chain reactiones
dc.subjectvirus titrationes
dc.titleDifferential Expression of Toll-like Receptors in Dendritic Cells of Patients with Dengue during Early and Late Acute Phases of the Diseasees
dc.identifier.bibliographicCitationTorres S,Hernández JC,Giraldo D,Arboleda M,Rojas M,Smit JM,Urcuqui S. Differential Expression of Toll-like Receptors in Dendritic Cells of Patients with Dengue during Early and Late Acute Phases of the Disease. PLoS Negl Trop Dis. 2013. 7. (2):e2060. .es
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