Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12494/41722
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Title: Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications
Author: Laranjeira M.S.
Carvalho Â.
Pelaez Vargas, Alejandro
Hansford D.
Ferraz M.P.
Coimbra S.
Costa E.
Santos-Silva A.
Fernandes M.H.
Monteiro F.J.
Email autor: alejandro.pelaezv@campusucc.edu.co
Issue Date: 2014
Keywords: Adhesion
Biological materials
Biomaterials
Cell adhesion
Cell culture
Coatings
Dental materials
Dental prostheses
Gene expression
Self assembly
Silica
Sol-gel process
Bacteria adhesions
hemolysis
Human gingival fibroblasts
Micro patterning
Microvascular endothelial cells
Soft lithography
Zirconia
Abstract: Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined methodology of sol-gel processing and soft lithography. The aim of the work was to compare the in vitro behavior of human gingival fibroblasts (HGFs) and human dermal microvascular endothelial cells (HDMECs) on three types of silica-coated zirconia surfaces: flat and micropatterned (with pillars and with parallel grooves). Our results showed that cells had a higher metabolic activity (HGF, HDMEC) and increased gene expression levels of fibroblast-specific protein-1 (FSP-1) and collagen type I (COL I) on surfaces with pillars. Nevertheless, parallel grooved surfaces were able to guide cell growth. Even capillary tube-like networks of HDMEC were oriented according to the surface geometry. Zirconia and silica with different topographies have shown to be blood compatible and silica coating reduced bacteria adhesion. All together, the results indicated that microstructured bioactive coating seems to be an efficient strategy to improve soft tissue integration on zirconia implants, protecting implants from peri-implant inflammation and improving long-term implant stabilization. This new approach of micropatterned silica coating on zirconia substrates can generate promising novel dental implants, with surfaces that provide physical cues to guide cells and enhance their behavior. © 2014 National Institute for Materials Science.
Type: Artículo
Citation: Laranjeira MS,Carvalho Â,Pelaez A,Hansford D,Ferraz MP,Coimbra S,Costa E,Santos A,Fernandes MH,Monteiro FJ. Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications. Sci Technol Adv Mater. 2014. 15. (2):p. 25001-25001. .
Other Identifiers: https://doi.org/10.15446/agron.colomb.v34n1supl.57341
Appears in Collections:Artículos Científicos

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