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Title: Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
Author: Carvalho A.
Pelaez Vargas, Alejandro
Hansford D.J.
Fernandes M.H.
Monteiro F.J.
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Issue Date: 2016
Keywords: Atomic force microscopy
Cell culture
Scanning electron microscopy
Stem cells
Surface treatment
Tissue engineering
Basal conditions
Biochemical stimuli
Bone tissue engineering
Extracellular matrices
Human bone marrow derived mesenchymal stem cells
Osteogenic differentiation
Silica thin films
Tissue implants
Thin films
silicon dioxide
cell culture
cell differentiation
mesenchymal stroma cell
reverse transcription polymerase chain reaction
tissue engineering
Cell Differentiation
Mesenchymal Stromal Cells
Reverse Transcriptase Polymerase Chain Reaction
Silicon Dioxide
Tissue Engineering
Abstract: A primary goal in bone tissue engineering is the design of implants that induce controlled, guided, and rapid healing. The events that normally lead to the integration of an implant into bone and determine the performance of the device occur mainly at the tissue-implant interface. Topographical surface modification of a biomaterial might be an efficient tool for inducing stem cell osteogenic differentiation and replace the use of biochemical stimuli. The main goal of this work was to develop micropatterned bioactive silica thin films to induce the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) only through topographical stimuli. Line and pillar micropatterns were developed by a combination of sol-gel/soft lithography and characterized by scanning electron microscopy, atomic force microscopy, and contact angle measurements. hMSCs were cultured onto the microfabricated thin films and flat control for up to 21 days under basal conditions. The micropatterned groups induced levels of osteogenic differentiation and expression of osteoblast-associated markers higher than those of the flat controls. Via comparison of the micropatterns, the pillars caused a stronger response of the osteogenic differentiation of hMSCs with a higher level of expression of osteoblast-associated markers, ALP activity, and extracellular matrix mineralization after the cells had been cultured for 21 days. These findings suggest that specific microtopographic cues can direct hMSCs toward osteogenic differentiation. © 2016 American Chemical Society.
Type: Artículo
Citation: Carvalho A,Pelaez A,Hansford DJ,Fernandes MH,Monteiro FJ. Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells. Langmuir. 2016. 32. (4):p. 1091-1100. .
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Appears in Collections:Artículos Científicos

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