Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12494/41616
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Title: Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia
Author: Vargas-Hernández G.
André M.R.
Faria J.L.M.
Munhoz T.D.
Hernandez Rodriguez, Milena Esney
Machado R.Z.
Tinucci-Costa M.
Email autor: milena.hernandez@campusucc.edu.co
Issue Date: 2012
Keywords: immunoglobulin G
RNA 16S
antibody detection
article
Babesia
Babesia vogeli
blood sampling
Colombia
controlled study
dog
Ehrlichia canis
geographic distribution
nonhuman
nucleotide sequence
parasite identification
phylogenetic tree
polymerase chain reaction
RNA sequence
sequence alignment
sequence analysis
serology
Animals
Babesia
Babesiosis
Colombia
Dog Diseases
Dogs
Ehrlichia canis
Ehrlichiosis
Phylogeny
Seroepidemiologic Studies
Anaplasmataceae
Animalia
Babesia
Babesia canis
Babesia sp
Canis familiaris
Ehrlichia canis
Ixodida
Piroplasmida
Rhipicephalus sanguineus
Abstract: Ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by Ehrlichia canis and Babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, Rhipicephalus sanguineus. The present work aimed to detect the presence of E. canis and Babesia sp. in 91 dog blood samples in Colombia, by molecular and serological techniques. We also performed sequence alignment to indicate the identity of the parasite species infecting these animals. The present work shows the first molecular detection of E. canis and B. vogeli in dogs from Colombia. Immunoglobulin-G (IgG) antibodies to E. canis and Babesia vogeli were found in 75 (82.4%) and 47 (51.6%) sampled dogs, respectively. Thirty-seven (40.6%) and 5 (5.5%) dogs were positive in PCR for E. canis and Babesia sp., respectively. After sequencing, amplicons showed 99% of identity with isolates of E. canis and B. vogeli. The phylogenetic trees based on 16S rRNA-Anaplasmataceae sequences and 18S rRNA-piroplasmid sequences supported the identity of the found E. canis and B. vogeli DNAs, respectively. The present work shows the first molecular detection of E. canis and B. vogeli in dogs in Colombia. © 2011 Elsevier B.V.
Type: Artículo
Citation: Vargas G,André MR,Faria JLM,Munhoz TD,Hernandez M,Machado RZ,Tinucci M. Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia. VET PARASITOL. 2012. 186. (3-4):p. 254-260. .
Other Identifiers: https://doi.org/10.1159/000371765
https://psicologiaysalud.uv.mx/index.php/psicysalud/index
Appears in Collections:Artículos Científicos

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