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dc.creatorZapata Builes, Wildeman-
dc.creatorAguilar-Jiménez W.-
dc.creatorFeng Z.-
dc.creatorWeinberg A.-
dc.creatorRusso A.-
dc.creatorPotenza N.-
dc.creatorEstrada H.-
dc.creatorRugeles M.T.-
dc.description.abstractDefensins, RNases and cytokines are present at mucosal barriers, main ports of HIV entry, and are potential mediators of the resistant phenotype exhibited by HIV-1-exposed seronegative individuals (HESN) during sexual exposure. We aimed to determine the role of soluble factors in natural resistance to HIV-1 infection. Vaginal/endocervical/oral mucosal samples were taken from 60 HESN, 60 seropositive (SP) and 61 healthy controls (HC). Human neutrophil peptide 1 (hNP-1), human beta defensin (hBD) 2 and 3, RNases, MIP-1ß and RANTES mRNA transcripts were quantified by qPCR and in vitro single-round, recombinant-based viral infectivity assay was used to evaluate the anti-HIV-1 activity of hBDs and RNases. HESN expressed significantly higher levels of hNP-1, hBDs mRNA in oral mucosa compared to HC (P < 0.05). In genital mucosa, significantly higher mRNA levels of MIP-1ß, RANTES and RNases were found in HESN compared to HC (P < 0.05). HBDs and RNases inhibit HIV-1 replication, particularly R5 at entry, reverse transcription and nuclear import of the viral life cycle. hNP-1, hBDs, MIP-1ß, RANTES and RNases, collectively could contribute to HIV-1 resistance during sexual exposure. Moreover, the inhibition of HIV-1 infection in vitro by hBDs and RNases suggests that they may be exploited as potential antiretrovirals. © 2015 Institut
dc.description.provenanceMade available in DSpace on 2021-12-16T22:15:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2016en
dc.publisherElsevier Masson SASes
dc.relation.ispartofMICROBES AND INFECTIONes
dc.subjectbeta defensin 2es
dc.subjectbeta defensin 3es
dc.subjectmacrophage inflammatory protein 1betaes
dc.subjectmessenger RNAes
dc.subjectn [4 [[[6,7 dihydro 2 (4 methylphenyl) 5h benzocyclohepten 8 yl]carbonyl]amino]benzyl] n,n dimethyl 2h tetrahydropyran 4 aminium chloridees
dc.subjectneutrophil peptide 1es
dc.subjectunclassified druges
dc.subjectimmunologic factores
dc.subjectantiviral activityes
dc.subjectCD4+ T lymphocytees
dc.subjectcontrolled studyes
dc.subjectdose responsees
dc.subjectHuman immunodeficiency virus 1es
dc.subjectHuman immunodeficiency virus 1 infectiones
dc.subjectin vitro studyes
dc.subjectin vivo studyes
dc.subjectinnate immunityes
dc.subjectlife cyclees
dc.subjectmajor clinical studyes
dc.subjectmouth mucosaes
dc.subjectpolymerase chain reactiones
dc.subjectpriority journales
dc.subjectprotein analysises
dc.subjectprotein expressiones
dc.subjectprotein functiones
dc.subjectquantitative analysises
dc.subjectreverse transcriptiones
dc.subjectsexual intercoursees
dc.subjectuterine cervix mucosaes
dc.subjectvagina mucosaes
dc.subjectviral tropismes
dc.subjectvirus entryes
dc.subjectvirus infectivityes
dc.subjectvirus inhibitiones
dc.subjectvirus loades
dc.subjectvirus replicationes
dc.subjectvirus resistancees
dc.subjectvirus straines
dc.subjectdisease resistancees
dc.titleIdentification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1es
dc.identifier.bibliographicCitationZapata W,Aguilar W,Feng Z,Weinberg A,Russo A,Potenza N,Estrada H,Rugeles MT. Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1. MICROBES AND INFECTION. 2016. 18. (3):p. 211-219. .es
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