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Title: Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
Author: Zapata Builes, Wildeman
Aguilar-Jiménez W.
Feng Z.
Weinberg A.
Russo A.
Potenza N.
Estrada H.
Rugeles M.T.
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Issue Date: 2016
Keywords: angiogenin
beta defensin 2
beta defensin 3
macrophage inflammatory protein 1beta
messenger RNA
n [4 [[[6,7 dihydro 2 (4 methylphenyl) 5h benzocyclohepten 8 yl]carbonyl]amino]benzyl] n,n dimethyl 2h tetrahydropyran 4 aminium chloride
neutrophil peptide 1
unclassified drug
immunologic factor
antiviral activity
CD4+ T lymphocyte
controlled study
dose response
Human immunodeficiency virus 1
Human immunodeficiency virus 1 infection
in vitro study
in vivo study
innate immunity
life cycle
major clinical study
mouth mucosa
polymerase chain reaction
priority journal
protein analysis
protein expression
protein function
quantitative analysis
reverse transcription
sexual intercourse
uterine cervix mucosa
vagina mucosa
viral tropism
virus entry
virus infectivity
virus inhibition
virus load
virus replication
virus resistance
virus strain
disease resistance
Abstract: Defensins, RNases and cytokines are present at mucosal barriers, main ports of HIV entry, and are potential mediators of the resistant phenotype exhibited by HIV-1-exposed seronegative individuals (HESN) during sexual exposure. We aimed to determine the role of soluble factors in natural resistance to HIV-1 infection. Vaginal/endocervical/oral mucosal samples were taken from 60 HESN, 60 seropositive (SP) and 61 healthy controls (HC). Human neutrophil peptide 1 (hNP-1), human beta defensin (hBD) 2 and 3, RNases, MIP-1ß and RANTES mRNA transcripts were quantified by qPCR and in vitro single-round, recombinant-based viral infectivity assay was used to evaluate the anti-HIV-1 activity of hBDs and RNases. HESN expressed significantly higher levels of hNP-1, hBDs mRNA in oral mucosa compared to HC (P < 0.05). In genital mucosa, significantly higher mRNA levels of MIP-1ß, RANTES and RNases were found in HESN compared to HC (P < 0.05). HBDs and RNases inhibit HIV-1 replication, particularly R5 at entry, reverse transcription and nuclear import of the viral life cycle. hNP-1, hBDs, MIP-1ß, RANTES and RNases, collectively could contribute to HIV-1 resistance during sexual exposure. Moreover, the inhibition of HIV-1 infection in vitro by hBDs and RNases suggests that they may be exploited as potential antiretrovirals. © 2015 Institut Pasteur.
Type: Artículo
Citation: Zapata W,Aguilar W,Feng Z,Weinberg A,Russo A,Potenza N,Estrada H,Rugeles MT. Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1. MICROBES AND INFECTION. 2016. 18. (3):p. 211-219. .
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Appears in Collections:Artículos Científicos

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