Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12494/41438
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Title: Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration
Author: Carvalho A.
Pelaez Vargas, Alejandro
Gallego-Perez D.
Grenho L.
Fernandes M.H.
De Aza A.H.
Ferraz M.P.
Hansford D.J.
Monteiro F.J.
Email autor: alejandro.pelaezv@campusucc.edu.co
Issue Date: 2012
Keywords: AFM
Bacterial adhesion
Bioactive nanoparticles
Biological characterization
Biological response
FTIR/ATR
Guided tissue regeneration
Mesenchymal stem cell
Micro patterning
Micro-texture
Microaggregates
Micropatterned
Micropatterned surface
Nano-HA
Nano-hydroxyapatite
S
mutans
SEM/EDS
Silica thin films
Soft-lithography
Streptococcus mutans
Surface modification techniques
Adhesion
Bacteria
Biological materials
Biomaterials
Cell culture
Contact angle
Dental prostheses
Flowcharting
Lithography
Silica
Sol-gel process
Sol-gels
Surfaces
Thin films
Aggregates
biomaterial
dental material
hydroxyapatite
nanomaterial
silicon dioxide
anisotropy
article
atomic force microscopy
bacterium adherence
biofilm
cell adhesion
cell count
cell proliferation
cell survival
chemistry
computer aided design
cytology
human
infrared spectroscopy
materials testing
mesenchymal stroma cell
methodology
phase transition
physiology
scanning electron microscopy
s
Abstract: Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration. Objective: The main goal of this study was to produce micro-fabricated SiO 2 surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans. Materials and methods: A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO 2 thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films. Results: Micropatterned SiO 2 surfaces with 0%, 1% and 5% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces. Significance: The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO 2 presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration. © 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
Type: Artículo
Citation: Carvalho A,Pelaez A,Gallego D,Grenho L,Fernandes MH,De Aza AH,Ferraz MP,Hansford DJ,Monteiro FJ. Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration. DENT MATER. 2012. 28. (12):p. 1250-1260. .
Other Identifiers: https://doi.org/10.1016/j.humimm.2018.04.016
http://www.fipen.edu.br/hermes1/index.php/hermes1/article/view/481
Appears in Collections:Artículos Científicos

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