Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12494/41414
Exportar a:
Title: Venom from Opisthacanthus elatus scorpion of Colombia, could be more hemolytic and less neurotoxic than thought
Author: Estrada-Gómez S.
Vargas Muñoz, Leidy Johana
Saldarriaga-Córdoba M.
Quintana Castillo, Juan Carlos
Email autor: leidy.vargasmu@campusucc.edu.co
Issue Date: 2016
Keywords: 4 nitro 3 octanoyloxy benzoic acid
benzoic acid
phospholipase A2
scorpion venom
serine proteinase
unclassified drug
hemolytic agent
neurotoxin
scorpion venom
arachnid
biochemistry
DNA fingerprinting
enzyme activity
hydrolysis
mass spectrometry
microbial activity
proteomics
venom
animal experiment
antimicrobial activity
Article
blood clotting
chemical analysis
Colombia
controlled study
enzyme activity
female
hemolysis
human
hydrolysis
molecular weight
mouse
neurotoxicity
nonhuman
Opisthacanthus elatus
reversed phase high performance liquid chromatography
scorpion
tandem mass spectrometry
animal
chemistry
high performance liquid chromatography
scorpion
Colombia
Mammalia
Opisthacanthus
Scorpiones
Animals
Chromatography
High Pressure Liquid
Colombia
Hemolytic Agents
Molecular Weight
Neurotoxins
Scorpion Venoms
Scorpions
Tandem Mass Spectrometry
Abstract: We report the first biochemical, biological, pharmacological and partial proteomic characterization studies of the Opisthancanthus elatus venom (Gervais, 1844) from Colombia. The Reverse Phase High-Performance Liquid Chromatography venom profile showed 28 main well-defined peaks, most eluting between 20 and 45min (18-30% of acetonitrile, respectively). High-resolution mass analysis indicates the presence of 106 components ranging from 806.59742Da to 16849.4139Da. O. elatus venom showed hemolytic activity and hydrolyzed the specific substrate BapNa suggesting the presence of proteins with serine-protease activity. Collected RP-HPLC fractions eluting at 52.6, 55.5, 55.8, 56.2, and 63.9min (PLA2 region between 33 and 40% of acetonitrile), showed hemolytic activity and hydrolyzed the synthetic substrate 4-nitro-3-octanoyloxy-benzoic acid, indicating the presence of compounds with phospholipases A2 activity. These RP-HPLC fractions, showed molecular masses values up to 13978.19546Da, corroborating the possible presence of the mentioned enzymes. Tryptic digestion and MS/MS analysis showed the presence of a phospholipase like fragment, similar to on described in other Opisthacanthus genus studies. No coagulant activity was observed. No larvicidal or antimicrobial activity was observed at concentrations evaluated. Lethal and toxic activity is expected at doses above 100mg/kg, no neurotoxic effects were detected at lower doses. In conclusion, O. elatus exhibits a venom with a predominant phospholipase A2 activity than thought; mammal's neurotoxic activity is expected above the 100mg/kg, which is very high compared to the venom from other neurotoxic scorpions. © 2015 Elsevier B.V.
Type: Artículo
Citation: Estrada S,Vargas LJ,Saldarriaga M,Quintana JC. Venom from Opisthacanthus elatus scorpion of Colombia, could be more hemolytic and less neurotoxic than thought. ACTA TROP. 2016. 153. p. 70-78. .
Other Identifiers: https://doi.org/10.1016/j.comptc.2018.04.016
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062630680&doi=10.1186%2fs12985-019-1136-6&partnerID=40&md5=5767cde0dd8645cd8bfab19f2d6f4fc8
Appears in Collections:Artículos Científicos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.