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Title: HIV-1-exposed seronegative individuals show alteration in TLR expression and pro-inflammatory cytokine production ex vivo: An innate immune quiescence status?
Author: Hernandez Lopez, Juan Carlos
St Laurent G.
Urcuqui-Inchima S.
Email autor:
Issue Date: 2016
Keywords: caspase recruitment domain protein 15
interleukin 10
interleukin 18
interleukin 1beta
interleukin 6
messenger RNA
pattern recognition receptor
retinoic acid inducible protein I
toll like receptor
toll like receptor 2
toll like receptor 4
toll like receptor 8
tumor necrosis factor alpha
caspase recruitment domain protein 15
DDX58 protein
DEAD box protein
Human immunodeficiency virus antibody
NOD2 protein
retinoic acid inducible protein I
toll like receptor
clinical article
cohort analysis
controlled study
cytokine production
dendritic cell
ex vivo study
Human immunodeficiency virus 1
Human immunodeficiency virus 1 infection
innate immunity
peripheral blood mononuclear cell
priority journal
protein blood level
protein expression
protein function
cell culture
Human immunodeficiency virus infection
Abstract: Pattern recognition receptors (PRRs) are involved in direct recognition of viruses, promoting cellular activation and the production of pro-inflammatory cytokines. However, despite the reduced systemic immune activation described in HIV-1-exposed seronegatives (HESNs), few studies have focused on determining the relationship between PRR expression and cytokine production. We have aimed here to evaluate the expression level of PRRs and cytokines in HESNs, HIV-1 patients and healthy donors. Basal PRR expression levels in PBMCs, dendritic cells (DCs) and monocytes, and plasma cytokine levels as well as the PRR ligand-induced cytokine productions were determined by flow cytometry, qPCR and ELISA. Higher TLR2/4 expression in DCs and monocytes from HESNs was observed. Nevertheless, TLR4/8, NOD2 and RIG-I mRNA levels were lower in PBMCs from HESNs than HIV-1-infected patients. Comparable IL-1ß, IL-18 and TNF-a mRNA levels were observed among the groups examined; however, at the protein level, production of IL-1ß, IL-6 and IL-10 was significantly lower in plasma from HESNs than from HIV-1-infected patients. Our results suggest that exposure to HIV-1 without infection could be associated with reduced basal pro-inflammatory responses. Further studies are required to define the cell subsets responsible for these differences and the role of PRRs on protection against HIV-1 infection. © 2015, Springer Science+Business Media New York.
Publisher: Humana Press Inc.
metadata.dc.type: Artículo
Citation: Hernandez JC,St G,I,Urcuqui S. HIV-1-exposed seronegative individuals show alteration in TLR expression and pro-inflammatory cytokine production ex vivo: An innate immune quiescence status?. IMMUNOL RES. 2016. 64. (1):p. 280-290. .
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Appears in Collections:Artículos Científicos

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