Please use this identifier to cite or link to this item: https://repository.ucc.edu.co/handle/20.500.12494/41257
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dc.creatorQuintero-Gil D.C.-
dc.creatorOspina M.-
dc.creatorOsorio-Benitez J.E.-
dc.creatorMartinez Gutierrez, Marlen-
dc.date.accessioned2021-12-16T22:15:23Z-
dc.date.available2021-12-16T22:15:23Z-
dc.date.issued2014-
dc.identifierhttps://doi.org/10.3855/jidc.3978-
dc.identifierhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85083570770&doi=10.5281%2fzenodo.3740111&partnerID=40&md5=a238ce45aeb72ff414c5df59c2a80b7e-
dc.identifier.issn20366590es
dc.identifier.urihttp://hdl.handle.net/20.500.12494/41257-
dc.description.abstractIntroduction: Different dengue virus (DENV) serotypes have been associated with greater epidemic potential. In turn, the increased frequency in cases of severe forms of dengue has been associated with the cocirculation of several serotypes. Because Colombia is a country with an endemic presence of all four DENV serotypes, the aim of this study was to evaluate the in vivo and in vitro replication of the DENV-2 and DENV-3 strains under individual infection and coinfection conditions. Methodology: C6/36HT cells were infected with the two strains individually or simultaneously (coinfection). Replication capacity was evaluated by RT-qPCR, and the effects on cell viability were assessed with an MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Additionally, Aedes aegypti mosquitoes were artificially fed the two strains of each serotype individually or simultaneously. The viral genomes were quantified by RT-qPCR and the survival of the infected mosquitoes was compared to that of uninfected controls. Results: In single infections, three strains significantly affected C6/36HT cell viability, but no significant differences were found in the replication capacities of the strains of the same serotype. In the in vivo infections, mosquito survival was not affected, and no significant differences in replication between strains of the same serotype were found. Finally, in coinfections, serotype 2 replicated with a thousandfold greater efficiency than serotype 3 did both in vitro and in vivo. Conclusions: Due to the cocirculation of serotypes in endemic regions, further studies of coinfections in a natural environment would further an understanding of the transmission dynamics that affect DENV infection epidemiology. © 2014 Quintero-Gil et al.es
dc.description.provenanceMade available in DSpace on 2021-12-16T22:15:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.format.extent884-876es
dc.publisherJournal of Infection in Developing Countrieses
dc.relation.ispartofJ INFECT DEV COUNTRes
dc.subjectAedes aegypties
dc.subjectarticlees
dc.subjectcell viabilityes
dc.subjectcontrolled studyes
dc.subjectdenguees
dc.subjectDengue virus 2es
dc.subjectDengue virus 3es
dc.subjectgenetic associationes
dc.subjectgenetic variabilityes
dc.subjectgenotypees
dc.subjecthumanes
dc.subjectmixed infectiones
dc.subjectnonhumanes
dc.subjectnucleotide sequencees
dc.subjectreal time polymerase chain reactiones
dc.subjectserotypinges
dc.subjectstrain differencees
dc.subjectvirus isolationes
dc.subjectvirus replicationes
dc.subjectvirus survivales
dc.subjectAedeses
dc.subjectanimales
dc.subjectcell linees
dc.subjectcell survivales
dc.subjectColombiaes
dc.subjectcytologyes
dc.subjectDengue viruses
dc.subjectdisease carrieres
dc.subjectgeneticses
dc.subjectisolation and purificationes
dc.subjectpathogenicityes
dc.subjectphysiologyes
dc.subjectsalivary glandes
dc.subjectserotypees
dc.subjectvirologyes
dc.subjectvirus replicationes
dc.subjectAedeses
dc.subjectAnimalses
dc.subjectCell Linees
dc.subjectCell Survivales
dc.subjectCoinfectiones
dc.subjectColombiaes
dc.subjectDengue Viruses
dc.subjectInsect Vectorses
dc.subjectSalivary Glandses
dc.subjectSerogroupes
dc.subjectVirus Replicationes
dc.titleDifferential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoeses
dc.typeArtículo-
dc.creator.mailmarlen.martinezg@campusucc.edu.coes
dc.identifier.bibliographicCitationQuintero DC,Ospina M,Osorio JE,Martinez M. Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes. J Infect Dev Ctries. 2014. 8. (7):p. 876-884. .es
dc.rights.accessRightsDesconocidoes
dc.description.orcid0000-0002-9429-0058es
Appears in Collections:Artículos Científicos

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