Please use this identifier to cite or link to this item: https://repository.ucc.edu.co/handle/20.500.12494/41257
Title: Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes
Author: Quintero-Gil D.C.
Ospina M.
Osorio-Benitez J.E.
Martinez Gutierrez, Marlen
Email autor: marlen.martinezg@campusucc.edu.co
Issue Date: 2014
Keywords: Aedes aegypti
article
cell viability
controlled study
dengue
Dengue virus 2
Dengue virus 3
genetic association
genetic variability
genotype
human
mixed infection
nonhuman
nucleotide sequence
real time polymerase chain reaction
serotyping
strain difference
virus isolation
virus replication
virus survival
Aedes
animal
cell line
cell survival
Colombia
cytology
Dengue virus
disease carrier
genetics
isolation and purification
pathogenicity
physiology
salivary gland
serotype
virology
virus replication
Aedes
Animals
Cell Line
Cell Survival
Coinfection
Colombia
Dengue Virus
Insect Vectors
Salivary Glands
Serogroup
Virus Replication
Abstract: Introduction: Different dengue virus (DENV) serotypes have been associated with greater epidemic potential. In turn, the increased frequency in cases of severe forms of dengue has been associated with the cocirculation of several serotypes. Because Colombia is a country with an endemic presence of all four DENV serotypes, the aim of this study was to evaluate the in vivo and in vitro replication of the DENV-2 and DENV-3 strains under individual infection and coinfection conditions. Methodology: C6/36HT cells were infected with the two strains individually or simultaneously (coinfection). Replication capacity was evaluated by RT-qPCR, and the effects on cell viability were assessed with an MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Additionally, Aedes aegypti mosquitoes were artificially fed the two strains of each serotype individually or simultaneously. The viral genomes were quantified by RT-qPCR and the survival of the infected mosquitoes was compared to that of uninfected controls. Results: In single infections, three strains significantly affected C6/36HT cell viability, but no significant differences were found in the replication capacities of the strains of the same serotype. In the in vivo infections, mosquito survival was not affected, and no significant differences in replication between strains of the same serotype were found. Finally, in coinfections, serotype 2 replicated with a thousandfold greater efficiency than serotype 3 did both in vitro and in vivo. Conclusions: Due to the cocirculation of serotypes in endemic regions, further studies of coinfections in a natural environment would further an understanding of the transmission dynamics that affect DENV infection epidemiology. © 2014 Quintero-Gil et al.
Publisher: Journal of Infection in Developing Countries
metadata.dc.type: Artículo
Citation: Quintero DC,Ospina M,Osorio JE,Martinez M. Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes. J Infect Dev Ctries. 2014. 8. (7):p. 876-884. .
Other Identifiers: https://doi.org/10.3855/jidc.3978
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85083570770&doi=10.5281%2fzenodo.3740111&partnerID=40&md5=a238ce45aeb72ff414c5df59c2a80b7e
Appears in Collections:Artículos Científicos

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