Publicación: Evaluación de la actividad antimicrobiana de la tetraciclina HCl in vitro sobre Streptococcus mutans y Actinomyces naeslundii en diferentes concentraciones y vehículos de dilución.
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Introduction The formation of the microbial biofilm is the main risk factor for caries and periodontal disease, its mechanical elimination minimizes the multiplication of pathogenic bacteria. Streptococcus mutans secondary colonizer, important for the progression of caries. Actinomyces naeslundii, secondary colonizer that favors coaggregation. Scientific evidence of tetracycline shows favorable results in terms of local and critical use. Its topical application by irrigation promotes the union to root surfaces in periodontal pockets improving the disposition in time, and supporting antimicrobial measures. Objective: To evaluate the antimicrobial activity of tetracycline HCl in vitro on Streptococcus mutans (ATCC 25175) and Actinomyces naeslundii (ATCC 19039) in three dilution vehicles, risks and times. Materials and methods: In vitro experimental, descriptive observational study on the effect of the antimicrobial activity of tetracycline HCl dissolved in sterile distilled water, 0.9% saline, 2% lidocaine epinephrine with a concentration of 125mg, 250mg and 500mg on Streptococcus mutans and Actinomyces naeslundii, using the Kelsey-Maurer technique; dilution vehicles were used as negative controls and chlorhexidine as a positive control, all assays were affected in triplicate; descriptive statistics are detailed to analyze the data. Results and Conclusion. Tetracycline HCl was found to have a 100% antimicrobial effect on Streptococcus mutans (ATCC 25175) and Actinomyces naeslundii (ATCC 19039), the receptor for tetracycline HCl concentrations, current media and times.