Publicación: Evaluación de la actividad antimicrobiana de la tetraciclina - HCL diluida en agua destilada, solución salina y lidocaina al 2% con epinefrina 1:80000 sobre Prevotella intermedia y Porphyromonas gingivalis, estudio in vitro.
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2020_BernalOrtizyPerdomo_evaluacion_actividad_antimicrobiana_tetraciclina_diluida_prevotella_intermedia_porphyromonas_gingivaligingivalis_estudioinvitro.pdf
2020_BernalOrtizyPerdomo_evaluacion_actividad_antimicrobiana_tetraciclina_diluida_prevotella_intermedia_porphyromonas_gingivaligingivalis_estudioinvitro-LicenciaUso.pdf
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Periodontal diseases are chronic inflammatory conditions of the gums, their etiology is associated with dysbiosis that occurs in the presence of pathogenic periodontal microorganisms and the host's exacerbated immune response that results in the development of an inflammatory process that leads to damage to the periodontal tissues. Currently, the complementary treatment of these diseases includes the use of topical or locally applied antibiotics. In this study, tetracyclines were chosen, which are high-spectrum antibiotics with bactericidal activity against Gram positive and Gram negative bacteria; however, there is no established standardization regarding the concentration and dilution form of the drug, which possibly affects their antimicrobial effectiveness. Objective: The antimicrobial activity of tetracycline HCL was evaluated in vitro on Prevotella intermedia (ATCC®25611 ™) and Porphyromonas gingivalis (ATCC® 33277 ™) in different dilution vehicles and different concentrations. Materials and methods: In vitro experimental study evaluating the antimicrobial activity of tetracycline on Prevotella intermedia and Porphyromonas gingivalis, diluted in distilled water, saline, and 2% lidocaine - epinephrine 1: 800,000, in different concentrations (125, 250, 500 mg) and times (30, 60, 120 seconds), using the Kelseymaurer test, variables such as tetracycline concentration, time and antimicrobial effect were evaluated. The statistical analysis of the data was carried out by applying measures of central tendency such as the average and standard deviation, using the Excel program. Results: It was concluded that tetracycline HCL dissolved in 2% lidocaine anesthesia with epinephrine 1: 80,000, distilled water and saline in concentrations of the antibiotic (125, 250, 500mg) and in times of exposure (30, 60, 120 seconds) showed antimicrobial activity on Prevotella intermedia and Porphyromonas gingivalis. Key Words: Tetracycline, Prevotella intermedia, Porphyromonas gingivalis, In vitro techniques