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dc.contributor.advisorLópez Moncayo, Luis Fernando-
dc.contributor.advisorLópez Ordoñez, Alexander-
dc.coverage.spatialPastoes
dc.creatorCarlosama Recalde, Luis Alberto-
dc.creatorPazmiño Riobamba, Juan Camilo-
dc.creatorVelásquez Rivera, Adriana Carolina-
dc.date.accessioned2020-07-07T15:42:10Z-
dc.date.available2020-07-07T15:42:10Z-
dc.date.issued2020-06-12-
dc.identifier.urihttp://hdl.handle.net/20.500.12494/18165-
dc.descriptionEvaluar los niveles de SP, CGRP, sus receptores NK-1 y CGRP-R en el ligamento periodontal de premolares inferiores después de la preparación del conducto radicular utilizando los sistemas Reciproc blue®, M-Two® y Plex V®. Se obtuvieron cincuenta muestras de ligamento periodontal, provenientes de premolares inferiores extraídos por razones ortodónticas, las cuales fueron aleatoriamente distribuidas a cinco grupos: un control negativo o sin instrumentar, un control positivo preparado con limas manuales, y 3 sistemas de preparación (Reciproc Blue®, M-Two® y Plex V®) (n= 10). Los tejidos se almacenaron a -80 °C, posteriormente se descongelaron en un baño frio y se volvieron a congelar con sus respectivos deshielos para desintegrar el tejido. Se realizaron tres ciclos de sonicación hasta que las muestras se homogeneizaron, luego se centrifugó y se recogió el sobrenadante para la detección de los marcadores a estudiar. Las muestras se procesaron para la prueba ELISA utilizando el principio ELISA-sándwich. Para cada proteína analizada, se calculó la media, mediana y la desviación estándar en cada grupo. Las pruebas ANOVA y Kruskal-Wallis, se realizaron de acuerdo con su comportamiento paramétrico o no paramétrico. Finalmente se exploraron diferencias entre grupos por medio de una prueba pos-hoc Tukey en normalidad o U Mann-Whitney, si no eran normales.es
dc.description.abstractAssess the levels of SP, CGRP, its NK-1 and CGRP-R receptors in the periodontal ligament of the lower premolars after root canal preparation using the Reciproc blue®, M-Two® and Plex V® systems. Fifty periodontal ligament samples were obtained from lower premolars extracted for orthodontic reasons, which were randomly distributed to five groups: a negative control or without instrumentation, a positive control prepared with manual files, and 3 preparation systems (Reciproc Blue® , M-Two® and Plex V®) (n = 10). The tissues were stored at -80 ° C, then thawed in a cold bath and refrozen with their respective thaws to disintegrate the tissue. Three sonication cycles were performed until the samples were homogenized, then centrifuged and the supernatant was collected to detect the markers to be studied. The samples were processed for the ELISA test using the ELISA-sandwich principle. For each analyzed protein, the mean, median and standard deviation were calculated in each group. The ANOVA and Kruskal-Wallis tests were performed according to their parametric or non-parametric behavior. Finally, differences between groups were explored by means of a Tukey test in normality or U Mann-Whitney, if they were not normal.es
dc.format.extent80 p.es
dc.publisherUniversidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Especialización en Endodoncia, Pastoes
dc.subjectNeuropéptidoses
dc.subjectInflamación neurogénicaes
dc.subjectLigamento periodontales
dc.subjectPeriodontitis apical sintomáticaes
dc.subjectSustancia Pes
dc.subjectPéptido relacionado con el gen de la calcitoninaes
dc.subjectReceptor NK-1es
dc.subjectCGRP-Res
dc.subjectReciproc blue®es
dc.subjectM-Two®es
dc.subjectPlex V®es
dc.subject.classificationTG 2020 ENFes
dc.subject.otherNeuropeptideses
dc.subject.otherNeurogenic inflammationes
dc.subject.otherPeriodontal ligamentes
dc.subject.otherSymptomatic apical periodontitises
dc.subject.otherSubstance Pes
dc.subject.otherPeptide related to the calcitonin genees
dc.subject.otherNK-1 receiveres
dc.subject.otherCGRP-Res
dc.subject.otherReciproc blue®es
dc.subject.otherM-Two®es
dc.subject.otherPlex V®es
dc.titleNiveles De Sustancia P, Péptido Relacionado Con El Gen De Calcitonina Y Sus Receptores Nk-1 Y Cgrp-R Posteriores A La Preparación De Conductos Radiculares Con Los Sistemas Reciproc Blue®, M-Two® Y Plex V®, En El Ligamento Periodontal De Premolares Inferiores Humanoses
dc.typeTrabajos de grado - Posgrados-
dc.rights.licenseAtribución – No comercial – Sin Derivares
dc.publisher.departmentPastoes
dc.publisher.programEnfermeríaes
dc.publisher.programEspecialización alta gerencia y economía solidariaes
dc.publisher.programEspecialización en Aprendizaje Escolar y sus Dificultadeses
dc.publisher.programEspecialización en derecho administrativo disciplinarioes
dc.description.tableOfContentsResumen. -- 1. Planteamiento del problema de investigación. -- 2. Justificación. -- 3. Marco referencial. -- 3.1. Respuesta inflamatoria en el ligamento periodontal. -- 3.2. Neuropéptidos. -- 3.3. Receptores. -- 3.4. Sistemas de preparación. -- 3.5. Liberación de neuropéptidos con sistemas rotatorios. -- 4. Estado del arte. -- 5. Objetivos. -- 5.1. Objetivo General. -- 5.2. Objetivos específicos. -- 6. Metodología. -- 6.1. Enfoque y tipo de estudio. -- 6.2. Tipo de estudio. -- 6.3. Área de estudio. -- 6.4. Población de estudio y muestra. -- 6.5. Criterios de selección. -- 6.6. Operacionalización de variables. -- 6.7. Aleatorización. -- 6.8. Enmascaramiento. -- 6.9. Calibración de operadores. -- 6.10. Recolección de datos y procedimientos clínicos. -- 6.11. Toma y procesamiento de muestras. -- 7. Resultados. -- 7.1. Análisis de datos sociodemográficos. -- 7.2. Análisis bivariado. -- 7.3. Análisis de neuropéptidos y sus receptores. -- 8. Discusión. -- 9. Conclusiones. -- 10. Recomendaciones. -- 11. Bibliografía. -- Anexos.es
dc.creator.mailluis.carlosama@campusucc.edu.coes
dc.creator.mailjuan.pazmino@campusucc.edu.coes
dc.creator.mailadrianac.velasquez@campusucc.edu.coes
dc.identifier.bibliographicCitationCarlosama, L. Pazmiño, J. Velásquez, A. (2020). Niveles De Sustancia P, Péptido Relacionado Con El Gen De Calcitonina Y Sus Receptores Nk-1 Y Cgrp-R Posteriores A La Preparación De Conductos Radiculares Con Los Sistemas Reciproc Blue®, M-Two® Y Plex V®, En El Ligamento Periodontal De Premolares Inferiores Humanos. [Tesis de pregrado, Universidad Cooperativa de Colombia]. Repositorio Institucional UCC.es
dc.rights.accessRightsopenAccesses
dc.publisher.editorUniversidad Cooperativa de Colombiaes
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