Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12494/15916
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Title: Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
Author: Vargas Muñoz, Leidy Johana
Estrada-Gomez, Sebastian
Caldas Cardoso, Fernanda
Quintana-Castillo, Juan Carlos
Saldarriaga-Cordoba, Monica Maria
Arenas Gómez, Claudia Marcela
Pineda, Sandy Steffany
Email autor: leidy.vargasmu@campusucc.edu.co
sebastian.estrada@udea.edu.co
f.caldascardoso@imb.uq.edu.au
juan.quintana@campusucc.edu.co
monysalda@gmail.com
claudia.arenas@udea.edu.co
sandy.spineda@gmail.com
metadata.dc.description.cvlac: https://scienti.colciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000580023
Issue Date: 27-Aug-2019
Keywords: theraphosidae
Pamphobeteus
Peptides
Disulfide-rich peptide (DRP)
Inhibitory cysteine knot (ICK)
Venomics
Transcriptome
Ion channels
Abstract: Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium influx assays, tandem mass spectrometry analysis (tMS/MS), and venom-gland transcriptome. rp-HPLC fractions of P. verdolaga venom showed activity on CaV2.2, CaV3.2, and NaV1.7 ion channels. Active fractions contained several peptides with molecular masses ranging from 3399.4 to 3839.6 Da. The tMS/MS analysis of active fraction displaying the strongest activity to inhibit calcium channels showed sequence fragments similar to one of the translated transcripts detected in the venom-gland transcriptome. The putative peptide of this translated transcript corresponded to a toxin, here named !-theraphositoxin-Pv3a, a potential ion channel modulator toxin that is, in addition, very similar to other theraphositoxins a ecting calcium channels (i.e., !-theraphotoxin-Asp1a). Additionally, using this holistic approach, we found that P. verdolaga venom is an important source of disulfide-rich proteins expressing at least eight superfamilies.
Program: Medicina
Headquarters: Medellín
Type: Artículo
Citation: Estrada-Gomez, S., Cardoso, F. C., Vargas-Muñoz, L. J., Quintana-Castillo, J. C., Arenas Gómez, C. M., Pineda, S. S. y Saldarriaga-Cordoba, M. M. (2019). Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels. Toxins 2019, 11, 496. Recuperado de:
Resource reference: https://www.mdpi.com/2072-6651/11/9/496
Appears in Collections:Medicina



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