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Title: Molecular analysis of genetic structure and isolation distance of primary malaria vector Anopheles darling in Colombia.
Author: Rosero Galindo, Carol Yovanna
González Obando, Ranulfo
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Issue Date: Aug-2014
Keywords: Malaria
Resume: Ponencia internacional
Abstract: BACKGROUND: Anopheles darlingi Root 1926 is a primary vector of malaria in the Neotropic region, a specie not just highly antropophilic but very efficient in transmitting plasmodium species and is considered to be most important vector in the Amazon region. The principal main of this study was determine the genetic structure of An. darling populations using microsatellites (STR) as genetic markers applied in 300 anofeline females which were collected from six populations distributed in occidental and oriental regions of Colombia. METHODS: DNA extraction was done with the cited protocol of Gonzales (2007) band using the Genomic PrepTM Cell and tissue Isolation commercial kits. We used the STR reported by Conn et al (2001). RESULTS: The analysis with STR proved a high genetic diversity and significant alterations from the Hardy-Weinberg balance. The greatest count in diversity was The Mitú,-Vaupés (Na=14, Ho= 0.520). On the other hand, the least count of genetic diversity was Pueblo Nuevo-Córdoba (Na=12, Ho= 0.457). The oriental region and the Mitú populations presented the highest number of provate alleles (Ap=30; Ap=13; Ap=9.), whit variations between 0.010-0.097. The AMOVA analysis show that the whole population underwent moderated genetic differentiation (Frt=0.063, p<0.05). The same differentiation was noticed (0.06<Fst>0.06, p<0.05) with five of the six included populations in this job and a low differentiation in the Margaritas-Santander area (Frt=0.02s3, p<0.05). CONCLUSIONS: Our results suggest a slight positive correlation which does not show a statistical significance between the geographic and genetic distance, probably suggesting that the moderated genetic differentiation found between the couples in populations not be explained for the hypothesis of separation by distance.
Program: Medicina
Headquarters: Pasto
Type: Artículo
CC Licence: Licencia CC
Appears in Collections:Medicina

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