Ciencias de la salud
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Examinando Ciencias de la salud por Programa "Medicina veterinaria y zootecnia"
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- ÍtemCoinfection of Zika with Dengue and Chikungunya virus(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga, 2021) Carrillo-Hernández, Marlen; Ruiz Saenz, Julian; Martínez Gutiérrez, MarlenThe expansion of Zika virus (ZIKV) and its arrival to many tropical and subtropical countries have allowed the existence of coinfections with other arboviruses that cocirculate in these regions. In this study, we describe the distribution of coinfection of ZIKV with other arboviruses, the detection methods used for the identification of these viruses, and the potential implications of these coinfections regarding the prognosis of patients with the associated disease symptoms. ZIKV coinfection is present in nine countries around the world; it is most prevalent in Brazil, followed by Colombia. The principal technique used to identify coinfections is RT-qPCR. Regarding clinical manifestations, no differences have been found between the clinical signs and symptoms presented by monoinfected patients and by coinfected patients. However, relevant research is currently limited, since most pertinent studies are case reports and small-scale cross-sectional studies. Therefore, the impact that coinfections have on either the severity or recovery of the associated disease remains unknown.
- ÍtemDifferences in the replicative capacities of clinical isolates of dengue virus in C6/36 cells and in urban populations of Aedes aegypti from Colombia, South America(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga, 2018-08) Martínez Gutierrez, Marlen; Quintero-Gil, Diana; Uribe-Yepes, Alexander; Ospina, Martha; Diaz, Francisco Javier
- ÍtemFactores que dificultan la inseminación artificial en la especie ovina y su correlación con las tasas de fertilidad, preñez y parto.(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga, 2019) caceres bautista, Daniela; Mogollón Waltero, Edgar MauricioObjective: To carry out a systematic literature reviews to clarify which are the main factors that hinder pregnancy by artificial insemination in sheep. Selection of articles: Articles related to morphometric parameters, AI techniques that go through the cervix (IAV, IAC, IATC) and laparoscopic AI, ways to preserve semen (fresh, refrigerated and frozen-thawed) and heat synchronization protocols , that within their results determine fertility, pregnancy or childbirth rates. Exclusion criteria: Articles written in languages other than Spanish, Portuguese and English, with species other than sheep, which do not describe the synchronization protocol, the presentation of the semen, the type of AI technique used, within its methodology and results. Fertility, pregnancy or childbirth rates, and items for which you would have to pay. Results: We selected 52 articles that met the inclusion criteria, of which 16 were related to morphometric parameters, cervical os and its correlation with age, and 36 were related to AI techniques, type of semen conservation, synchronization protocols and their correlation with fertility, pregnancy and calving rates. Conclusion: This work made it possible to clarify that the main factors that make pregnancy difficult for AI in sheep are the age of the animals, the morphometric parameters of the cervix, the type of AI technique and the presentation of the semen.
- ÍtemIdentificación de compuestos antiarbovirus mediante bioprospección de plantas nativas colombianas de la familia solanaceae y análisis metabolómico de la respuesta celular frente al efecto antiviral(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga, 2021-04) Jimenez Posada, Erika; Robledo, Sara; Martínez Gutiérrez, Marlen
- ÍtemIn Vitro and In Silico Anti-Arboviral Activities of Dihalogenated Phenolic Derivates of L-Tyrosine(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaria y Zootecnia, Bucaramanga, 2021-06) Loaiza Cano, Vanessa; Mansalve Escudero, Laura; Pastrana Restrepo, Manuel; Quintero Gil, Diana Carolina; Pulido Muñoz, Sergio; Galeano, Elkin; Zapata, Wildeman; MARTINEZ GUTIERREZ, MARLENDespite the serious public health problem represented by the diseases caused by dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) viruses, there are still no specific licensed antivirals available for their treatment. Here, we examined the potential anti-arbovirus activity of ten di-halogenated compounds derived from L-tyrosine with modifications in amine and carboxyl groups. The activity of compounds on VERO cell line infection and the possible mechanism of action of the most promising compounds were evaluated. Finally, molecular docking between the compounds and viral and cellular proteins was evaluated in silico with Autodock Vina®, and the molecular dynamic with Gromacs®. Only two compounds (TDC-2M-ME and TDB-2M-ME) inhibited both ZIKV and CHIKV. Within the possible mechanism, in CHIKV, the two compounds decreased the number of genome copies and in the pre-treatment strategy the infectious viral particles. In the ZIKV model, only TDB-2M-ME inhibited the viral protein and demonstrate a virucidal effect. Moreover, in the U937 cell line infected with CHIKV, both compounds inhibited the viral protein and TDB-2M-ME inhibited the viral genome too. Finally, the in silico results showed a favorable binding energy between the compounds and the helicases of both viral models, the NSP3 of CHIKV and cellular proteins DDC and β2 adrenoreceptor.
- ÍtemIn vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga, 2019-11) Martinez Gutierrez, Marlen; Trujillo-Correa, Andrea Isabel; Quintero-Gil, Diana Carolina; Diaz-Castillo, Fredyc; Quiñones, Winston; Robledo, Sara M
- ÍtemIndole alkaloids inhibit zika and chikungunya virus infection in different cell lines(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga, 2021-08) Monsalve Escudero, Laura; Loaiza Cano, Vanessa; Pajaro Gonzalez, Yina; Oliveros Diaz, Andres; Diaz Castillo, Fredyc; Quiñones, Wiston; Robledo, Sara; Martínez Gutiérrez, MarlenAbstract Background In recent years, an increase in the occurrence of illnesses caused by two clinically- important arboviruses has been reported: Zika virus (ZIKV) and Chikungunya virus (CHIKV). There is no licensed antiviral treatment for either of the two abovementioned viruses. Bearing in mind that the antiviral effect of indole alkaloids has been reported for other arboviral models, the present study proposed to evaluate the antiviral in vitro and in silico effects of four indole alkaloids on infections by these two viruses in different cell lines. Methods The antiviral effects of voacangine (VOAC), voacangine-7-hydroxyindolenine (VOAC-OH), rupicoline and 3-oxo voacangine (OXO-VOAC) were evaluated in Vero, U937 and A549 cells using different experimental strategies (Pre, Trans, Post and combined treatment). Viral infection was quantified by different methodologies, including infectious viral particles by plating, viral genome by RT-qPCR, and viral protein by cell ELISA. Moreover, molecular docking was used to evaluate the possible interactions between structural and nonstructural viral proteins and the compounds. The results obtained from the antiviral strategies for each experimental condition were compared in all cases with the untreated controls. Statistically significant differences were identified using a parametric Student’s t-test. In all cases, p values below 0.05 (p < 0.05) were considered statistically significant. Results In the pre-treatment strategy in Vero cells, VOAC and VOAC-OH inhibited both viral models and OXO-VOAC inhibited only ZIKV; in U937 cells infected with CHIKV/Col, only VOAC-OH inhibited infection, but none of the compounds had activity in A549 cells; in U937 cells and A549 cells infected with ZIKV/Col, the three compounds that were effective in Vero cells also had antiviral activity. In the trans-treatment strategy, only VOAC-OH was virucidal against ZIKV/Col. In the post-treatment strategy, only rupicoline was effective in the CHIKV/Col model in Vero and A549 cells, whereas VOAC and VOAC-OH inhibited ZIKV infection in all three cell lines. In the combined strategy, VOAC, VOAC-OH and rupicoline inhibited CHIKV/Col and ZIKV/Col, but only rupicoline improved the antiviral effect of ZIKV/Col-infected cultures with respect to the individual strategies. Molecular docking showed that all the compounds had favorable binding energies with the structural proteins E2 and NSP2 (CHIKV) and E and NS5 (ZIKV). Conclusions The present study demonstrates that indole alkaloids are promising antiviral drugs in the process of ZIKV and CHIKV infection; however, the mechanisms of action evaluated in this study would indicate that the effect is different in each viral model and, in turn, dependent on the cell line.
- ÍtemSARS-CoV-2 Clinical Outcome in Domestic and Wild Cats: A Systematic Review(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga, 2021-07) Giraldo Ramirez, Sebastian; Rendon Marin, Santiago; Jaimes, Javier Andres; Martínez Gutiérrez, Marlen; Ruiz Saenz, JulianRecently, it has been proved that SARS-CoV-2 has the ability to infect multiple species. This work was aimed at identifying the clinical signs of SARS-CoV-2 infection in domestic and wild felids. A PRISMA-based systematic review was performed on case reports on domestic and wild cats, reports on experimental infections, case reports in databases, preprints and published press releases. Descriptive statistical analysis of the data was performed. A total of 256 articles, 63 detailed official reports and 2 press articles on SARS-CoV-2 infection in domestic and wild cats were analyzed, of which 19 articles and 65 reports were finally included. In domestic cats, most cats’ infections are likely to be asymptomatic, and 46% of the reported infected animals were symptomatic and predominantly presented respiratory signs such as sneezing and coughing. In wild felines, respiratory clinical signs were most frequent, and up to 96.5% of the reported affected animals presented coughing. It is noteworthy that, to date, symptomatic animals with SARS-CoV-2 infection have been reported to belong to two different subfamilies (Phanterinae and Felinae), with up to five different felid species affected within the Felidae family. Reported results evince that the signs developed in felids show similar progression to those occurring in humans, suggesting a relationship between the viral cycle and target tissues of the virus in different species. While viral transmission to humans in contact with animal populations has not been reported, spill-back could result in the emergence of immune-escape mutants that might pose a risk to public health. Despite the clear results in the identification of the typical clinical picture of SARS-CoV-2 infection in felines, the number of detailed academic reports and papers on the subject is scarce. Therefore, further description of these cases will allow for more accurate and statistically robust clinical approaches in the future
- ÍtemThe antiviral and virucidal activities of voacangine and structural analogs extracted from Tabernaemontana cymosa depend on the Dengue virus strain(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga, 2021-06) Monsalve Escudero, Laura; Loaiza Cano, Vanessa; Zapata, Maria; Quintero Gil, Diana Carolina; Hernandez Mira, Estiven; Pajaro Gonzalez, Yina; Oliveros Diaz, Andres; Diaz castillo, Fredyc; Quiñones, Wiston; Robledo, Sara; MARTINEZ GUTIERREZ, MARLENCurrently, no specific licensed antiviral exists for treating the illness caused by dengue virus (DENV). Therefore, the search for compounds of natural origin with antiviral activity is an important area of research. In the present study, three compounds were isolated and identified from seeds of Tabernaemontana cymosa plants. The in vitro antiviral effect of those compounds and voacangine against different DENV strains was assessed using different experimental approaches: compounds added before the infection (Pre), at the same time with the virus (Trans), after the infection (Post) or compounds present in all moments of the experiment (Pre-Trans-Post, Combined treatment). In silico studies (docking and molecular dynamics) were also performed to explain the possible antiviral mechanisms. The identified compounds were three structural analogs of voacangine (voacangine-7-hydroxyindolenine, rupicoline and 3-oxo-voacangine). In the Pre-treatment, only voacangine-7-hydroxyindolenine and rupicoline inhibited the infection caused by the DENV-2/NG strain (16.4% and 29.6% infection, respectively). In the Trans-treatment approach, voacangine, voacangine-7-hydroxyindolenine and rupicoline inhibited the infection in both DENV-2/NG (11.2%, 80.4% and 75.7% infection, respectively) and DENV-2/16681 infection models (73.7%, 74.0% and 75.3% infection, respectively). The latter strain was also inhibited by 3-oxo-voacangine (82.8% infection). Moreover, voacangine (most effective virucidal agent) was also effective against one strain of DENV-1 (DENV-1/WestPac/74) and against the third strain of DENV-2 (DENV-2/S16803) (48.5% and 32.4% infection, respectively). Conversely, no inhibition was observed in the post-treatment approach. The last approach (combined) showed that voacangine, voacangine-7-hydroxyindolenine and rupicoline inhibited over 90% of infections (3.5%, 6.9% and 3.5% infection, respectively) of both strains (DENV-2/NG and DENV-2/16681). The free energy of binding obtained with an in silico approach was favorable for the E protein and compounds, which ranged between −5.1 and −6.3 kcal/mol. Finally, the complex formed between DENV-2 E protein and the best virucidal compound was stable for 50 ns. Our results show that the antiviral effect of indole alkaloids derived from T. cymose depends on the serotype and the virus strain.
- ÍtemYellow fever reemergence in Venezuela – Implications for international travelers and Latin American countries during the COVID-19 pandemic(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga, 2021-10) Rodriguez-Morales, Alfonso; Bonilla, Diana; Suarez, Jose Antonio; Franco Paredes, Carlos; Forero Peña, David; Mattar, Salim; Villamil, Wilmer; RUIZ SAENZ, JULIAN; Cardona Ospina, Jaime Andres; Figueroa, Manuel; Sierra carrero, Leandro; Risquez, Alejandro; Cimerman, Sergio; Valero cedeño, Nereida; Cabrera, Maritza; Robaina Barrios, Andrea; Lopez Diaz, Luis; Barbella, Rosa; Navas, Rosa; Diaz Quijano, Fredi; Carrero, Yenddy; Pineda, Anishmedia; Brito, Maximo; Savio Larriera, Eduardo; MARTINEZ GUTIERREZ, MARLEN; Maquera Afaray, Julio; Solarte Portilla, Marco; Henao Martinez, Andres; Ortiz Martinez, Yeimer; Souza Chaves, Tania; Orduna, Tomas; Lepetic, Alejandro; Macchi, Alejandra; Verbanaz, Sergio; Perret, Cecilia; Echazarreta, Sofia; Lloveraz, Susana; Gallego, Viviana; Navarro, Juan Carlos; Paniz Mondolfi, AlbertoIn the last few years, Venezuela has been the epicentre for multiple concurrent epidemics (syndemics) of emerging and reemerging infectious and tropical diseases. These events have severe implications for public health control efforts in Latin American and other regions due to the latent threat of case importation. At the top of the list, the resurgence of vector-borne diseases (VBD), such as malaria and dengue pose substantial challenges for the region . On the other hand, the reemergence of vaccine-preventable diseases (VPD) due to low immunization coverage has led to new and multiple outbreaks of measles, diphtheria and other vaccine-preventable infections that are once again affecting many populations.