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Examinando Investigación por Programa "Odontología"
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- ÍtemBacteriome Identified by Next-Generation Sequencing in Saliva, Dental Plaque, and Tumor Tissue of Patients with Oral Squamous Cell Carcinoma(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Odontología, Bogotá, 2021-06-07) ERIRA, ALVEIRO; ERIRA, ALVEIRO; GARCIA, ADRIANA; GAMBOA, FREDY; TOBAR, FABIAN; CHALA, ANDRES; CID, ANGEL; MORENO, ANDREI; MUÑOZ, ELIANA; ERIRA, ALVEIROEl carcinoma oral de células escamosas (OSCC) es el sexto cáncer más común en el mundo, y el microbioma bacteriano se ha considerado un riesgo factor que podría desempeñar un papel importante en la carcinogénesis. Objetivo: Se realizó un estudio de bacterioma mediante secuenciación de próxima generación en muestras de placa dental, saliva y tumor de 10 pacientes con OSCC y se comparó con bacterioma en placa dental y saliva de 10 pacientes sin COCE. Métodos: El ADN se extrajo de todas las muestras y se secuenció con la tecnología MiSeq™ de Illumina. Se realizaron análisis bioinformáticos para evaluar calidad de secuencia, diversidad alfa y beta, análisis de varianza bidireccional (p <0,05) y análisis de componentes principales. Después de establecer Se realizaron perfiles bacterianos asociados a cada muestra y población, comparaciones intragrupo e intergrupo. Para la identificación de bacterias compatibles con procesos de eubiosis y disbiosis, se realizó un cribado en función de la frecuencia de aparición en todas las muestras de pacientes con y sin OSCC. Por último, se calcularon la frecuencia, la media, la desviación estándar, la Chi-cuadrado y la prueba de Mann Whitney. Resultados: De las 1.231 bacterias identificadas en las poblaciones objeto de estudio, se seleccionaron 45 especies bacterianas, de las cuales 34 eran compatibles con eubiosis, y 11 eran compatibles con disbiosis. Entre las bacterias compatibles con la eubiosis se encontraban especies de Lactobacillus y Streptococcus, Chromobacterium violaceum, Enterobacter asburiae, Mycobacterium chubuense, Mycoplasma penetrans y Brachyspira intermedia. Entre la especies asociadas con disbiosis, Providencia stuartii, Capnocytophaga canimorsus, Legionella pneumophila y Mycoplasma hominis fueron Incapaz. Conclusión: Treinta y cuatro especies bacterianas pueden estar asociadas con eubiosis o estados saludables y 11 especies bacterianas podrían estar asociadas con disbiosis o estado patógeno, OSCC.
- ÍtemDifferential Regulation of the EGFR/PI3K/AKT/PTEN Pathway between Low-and High-Grade Gliomas(Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Odontología, Bogotá, 2021-12-18) ERIRA, ALVEIRO; ERIRA, ALVEIRO; ARBOLEDA BUSTOS, GONZALO H.; VELANDIA, FERNANDO; ZUBIETA, CAMILO; PENAGOS, JOSÉ; ERIRA, ALVEIROGliomas represent 70% of all central system nervous tumors and are classified according to the degree of malignancy as low- or high-grade. The permanent activation of the EGFR/PI3K/AKT pathway by various genetic or post-translational alterations of EGFR, PI3KCA, and PTEN has been associated with increased proliferation and resistance to apoptosis. The present study aimed to analyze the molecular/genetic changes in the EGFR/PI3K/AKT/PTEN pathway between low-grade and high-grade gliomas in a sample of Colombian patients. A total of 30 samples were tested for PI3K and PTEN mutations, EGFR, PI3K, and AKT gene amplification, AKT, PI3K, BAX, Bcl2 expression levels, and phosphorylation of AKT and PTEN, EGFR and/or PI3K gene amplification was found in 50% of low-grade and 45% of high-grade ones. AKT amplification was found in 25% of the low-grade and 13.6% of the high-grade. The expression of PI3K, AKT, Bcl2, and BAX was increased particularly to a high degree. AKT phosphorylation was found in 66% of low-grade and 31.8% of high-grade. Increased phosphorylation of PTEN was found in 77% low-grade and 66% high-grade. Our results indicate that alterations in the EGFR/PI3K/AKT/PTEN pathway could be important in the initiation and malignant progression of this type of tumor.
- ÍtemDoes high sugar intake really alter the oral microbiota?: A systematic review(Universidad Cooperativa de Colombia, 2022-08-09) Universidad Cooperativa de Colombia; Angarita-Díaz, María del Pilar; Fong, Cristian; Claudia, Bedoya-Correa; Claudia, Cabrera-Arango; Clinical and Experimental Dental Research
- ÍtemEvaluation of possible biomarkers for caries risk in children 6 to 12 years of age(Universidad Cooperativa de Colombia, 2021-08-17) Universidad Cooperativa de Colombia; Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana; Angarita-Díaz, María del Pilar; Simon-Soro, Aurea; Forero, Diana; Balcázar, Felipe; Sarmiento, Luisa; Romero, Erika; Mira, AlexBackground: Electrolytes, proteins, and other salivary molecules play an important role in tooth integrity and can serve as biomarkers associated with caries. Objective: To determine the concentration of potential biomarkers in children without caries (CF) and children with caries (CA). Methods: Unstimulated saliva was collected, and the biomarkers quantified in duplicate, using commercial Enzyme Linked Immunosorbent Assay (ELISA) kits to determine IgA, fibronectin, cathelicidin LL-37, and statherin levels, as well as colorimetric tests to detect formate and phosphate. Results: Significantly higher concentrations of statherin was detected in the CF group (Median: 94,734.6; IQR: 92,934.6–95,113.7) compared to the CA2 group (90,875.0; IQR: 83,580.2–94,633.4) (p = 0.03). Slightly higher median IgA (48,250.0; IQR: 31,461.9–67,418.8) and LL-37 levels (56.1; IQR 43.6–116.2) and a lower concentration of formate were detected in the CF group (0.02; IQR 0.0034–0.15) compared to the group with caries (IgA: 37,776.42; IQR: 33,383.9–44,128.5; LL-37: 46.3; IQR: 40.1011–67.7; formate: 0.10; IQR: 0.01–0.18), but these differences were not statistically significant. Conclusion: The fact that these compounds have been identified as good markers for caries among European adults highlights the difficulty of identifying universal biomarkers that are applicable to all ages or to different populations.
- ÍtemImpact of a virtual learning environment on the conscious prescription of antibiotics among Colombian dentists(Universidad Cooperativa de Colombia, 2022-01-28) Universidad Cooperativa de Colombia; Angarita-Díaz, María del Pilar; Bernal-Cepeda, Lilia; Bastidas-Legarda, Leidy; Forero-Escobar, Diana; Ricaurte-Avendaño, Angélica; Mora-Reina, Julián; Vergara-Mercado, Martha; Herrera-Herrera, Alejandra; Rodriguez-Paz, Martha; Cáceres- Matta, Sandra; Fortich-Mesa, Natalia; Ochoa-Acosta, Emilia M.; Plos OneAppropriate antibiotic prescription contributes to reducing bacterial resistance; therefore, it is critical to provide training regarding this challenge. The objective of this study was to develop a virtual learning environment for antibiotic prescription and to determine its impact on dentists’ awareness, attitudes, and intention to practice. First, the learning content on multimedia resources was developed and distributed into three challenges that participants had to overcome. Then, a quasi-experimental study was performed in which the virtual learning environment was implemented on dentists from seven Colombian cities. The median of correct answers and the levels of awareness, attitudes, and intention to practice were compared before, immediately after, and 6-months post-intervention. Wilcoxon signed-rank and McNemar’s tests were used to determine the differences. A total of 206 participants who finished the virtual learning environment activities exhibited a favorable and statistically significant impact on the median of correct answers of awareness (p < 0.001), attitudes (p < 0.001), and intention to practice (p = 0.042). A significant increase occurred in the number of participants with a high level of awareness (p < 0.001) and a non-significant increase in participants with high levels of attitudes (p = 0.230) and intention to practice (p = 0.286). At 6 months, the positive effect on the median of correct answers on awareness and intention to practice persisted (p < 0.001); however, this was not evident for attitudes (p = 0.105). Moreover, there was a significant decrease in the number of participants who showed low levels of awareness (p = 0.019) and a slight increase in those with high levels of the same component (p = 0.161). The use of a virtual learning environment designed for dentists contributed to a rapid improvement in awareness and intention to practice antibiotic prescription; however, their attitudes and information retention need reinforcement.
- ÍtemSugar in ultraprocessed foods in Latin American capitals: From dentistry perspective(Universidad Cooperativa de Colombia, 2023-02-04) Universidad Cooperativa de Colombia; Angarita-Díaz, María del Pilar; Lopez-Ramos, Roxana Patricia; Tipan-Asimbaya , Lissette; Ruiz-Gomez, Adiela; Gonzalez-Bernal, María Alejandra; Vicuña-Huaqui, Luis Antonio; Professor Jan Frank
- ÍtemThe efect of commercial functional food with probiotics on microorganisms from early carious lesions(Universidad Cooperativa de Colombia, Medellín. Odontología, 2020-07) Angarita‑Díaz, María del Pilar; Arias, Johanna C; Bedoya‑Correa, Claudia; Cepeda, María J; Arboleda, María F; Chacón, Juan M; Leal, Yenny; Angarita‑Díaz, María del PilarCaries rates in school-age children are still high enough to be the cause of serious concern for health systems in diferent countries. The biotechnology strategies studied to decrease these rates include the consumption of probiotics—available via a variety of functional foods obtainable on the market— that are able to inhibit bacteria associated with this disease. In this vein, the purpose of this study was to determine the efect of these foods on the growth of microorganisms in early carious lesions in children aged between 6 and 12. In the frst phase, an agar well difusion method was applied to selected foods, available in supermarkets, which contain probiotics that have already been shown to inhibit Streptococcus mutans (ATCC 25175), and to lower the pH in liquid culture media. In a second phase, these foods (n= 4) were examined in terms of their ability to inhibit the microorganisms in contact with early carious lesions in children and to reduce the pH of mixed cultures combined with the food. The results revealed that, of the foods tested, three inhibit the growth of microorganisms in carious lesions and, at the same time, lower the pH of the culture by more than 2.5 units. The food with the highest inhibitory capacity (14 mm, IQR 13–14) showed a similar efect among patients (P> 0.05), which together with the fact that its sugar concentration is less than 10%, makes it an ideal candidate for clinical study.