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Title: Use of the mitochondrial serine transfer RNA (UCN) of Lutzomyia columbiana (Diptera, Psychodidae) in phylogeographic studies
Author: Rosero Galindo, Carol Yovanna
García López, Juan Pablo
Montenegro Coral, Franco Andrés
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Issue Date: Aug-2014
Keywords: vectors of Leishmania sp
diseases in human
genetic diversity
mitochondrial gene
Resume: Ponencia Internacional
Abstract: BACKGROUND: Sand flies are small diptera of Psychodidae family of medical importance due to their role like vectors of Leishmania sp., Acording to the low knowledge of molecular characteristics of this specie in our region, the principal main of this study was evaluate the genetic diversity of mitochondrial gene encoding serine (ser-tRNA), in natural populations of Lu. columbiana in Nariño, southern Colombia. METHODS: The species status was confirmed by using the taxonomic key generated by Young and Duncan (1994) and Galati (2003). The genetic analysis was performed by amplification by PCRof gene ser-tRNA using the primers identified by Ready et al (1997), which have as target sequence the 3' end by the 3' Cyt b'subunit 1 gene of NADH dehydrogenase (NAD). The same conditions for PCR reported by Perez (2008) were used. RESULTS: The ser-tRNA´s gene amplification was necessary to modify the PCR conditions reported by Pérez (2008). In a final volume of 12.5 ul used 1X PCR buffer, 1.5 mM MgCl2, 1.5 U of Taq polymerase ( Promega GoTaq Kit), 0.2 mm dNTP's (Promega ), 0.3 um of each primer and 6ul of DNA. The ser-tRNA´s gene consisted of 67 base pairs. The sequencing analysis show value of S:0 to number of polymorphic sites, Eta:0 to total number of mutations, h:1 to number of haplotypes, and Haplotype (gene) diversity, both variance of haplotype diversity and standard deviation of haplotype diversity values below 0. CONCLUSIONS: The number of haplotypes found in populations of Lu. columbiana exhibited genetic variance possibly mainly due to geographical diferences, however is necessary increase the number of individuals analyzed to confirm the utility of the gene in phylogeographic studies.
Program: Medicina
Headquarters: Pasto
Publisher: Grupo Interdisciplinario de Investigación en Salud-Enfermedad, Facultad de Medicina, Universidad Cooperativa de Colombia, 520002 Pasto, Colombia.
Type: Ponencia
CC Licence: Licencia CC
Appears in Collections:Medicina

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