Please use this identifier to cite or link to this item: http://repository.ucc.edu.co/handle/ucc/1374
Title: Extracts of renealmia alpinia (Rottb.) MAAS protect against lethality and systemic hemorrhage induced by bothrops asper venom: insights from a model with extract administration before venom injection
Author: Quintana Castillo, Juan Carlos
Patiño, Arley Camilo
Gutiérrez, José María
Rucavado, Alexandra
Benjumea, Dora María
Pereañez, Jaime Andrés
Email autor: juan.quintanac@campusucc.edu.co
Issue Date: 30-Apr-2015
Advisor / Validator: Patiño, Arley Camilo
Quintana, Juan Carlos
Keywords: Micropropagated plants;Renealmia alpinia;Snake venoms;Metalloproteinase;Serine proteinase;Anti-venom activity
Abstract: Renealmia alpinia (Rottb.) MAAS, obtained by micropropagation (in vitro) and wild forms have previously been shown to inhibit some toxic activities of Bothrops asper snake venom if preincubated before injection. In this study, assays were performed in a murine model in which extracts were administered for three days before venom injection. R. alpinia extracts inhibited lethal activity of B. asper venom injected by intraperitoneal route. Median Effective Dose (ED50) values were 36.6 ± 3.2 mg/kg and 31.7 ± 5.4 mg/kg (p > 0.05) for R. alpinia wild and in vitro extracts, respectively. At a dose of 75 mg/kg, both extracts totally inhibited the lethal activity of the venom. Moreover, this dose prolonged survival time of mice receiving a lethal dose of venom by the intravenous route. At 75 mg/kg, both extracts of R. alpinia reduced the extent of venom-induced pulmonary hemorrhage by OPEN ACCESS Toxins 2015, 7 1533 48.0% (in vitro extract) and 34.7% (wild extract), in agreement with histological observations of lung tissue. R. alpinia extracts also inhibited hemorrhage in heart and kidneys, as evidenced by a decrease in mg of hemoglobin/g of organ. These results suggest the possibility of using R. alpinia as a prophylactic agent in snakebite, a hypothesis that needs to be further explored.
Program: Medicina
Headquarters: Medellín
Publisher: Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000
Type: Artículo
CC Licence: Licencia CC
Citation: Quintana Castillo, J. C., Patiño, A. C., Gutiérrez, J.M., Rucavado, A., Benjumea, D. M., & Pereañez, J. A. & (2015). Extracts of renealmia alpinia (Rottb.) MAAS protect against lethality and systemic hemorrhage induced by bothrops asper venom: insights from a model with extract administration before venom injection. Open Access Toxins, 7(5), 1-12.
Appears in Collections:Medicina

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