Please use this identifier to cite or link to this item: http://repository.ucc.edu.co/handle/ucc/1192
Title: HIV-1-derived single-stranded RNA acts as activator of human neutrophils
Author: Giraldo, Diana M.
Hernández, Juan C.
Urucqui Inchima, Silvio
Email autor: juanc.hernandezl@campusucc.edu.co
Issue Date: Oct-2016
Advisor / Validator: Hernández, Juan C.
Keywords: HIV-1;Neutrophils
Abstract: Neutrophils are key effector cells of the innate immune system and are involved in the host defense against invading pathogens such as viruses. Recently, it was reported that HIV-1-neutrophil interaction triggers neutrophil activation and promotes expression of Toll-like receptors (TLR). Here, we assessed the role of single-stranded RNA40 (ssRNA40) derived from HIV-1 in neutrophil activation. We observed functional activation of neutrophils in response to HIV-1-derived ssRNA40 based on the expression of TLR7/8, RIG-I and MDA5, induction of cytokines (IL-6 and TNF-α) and the production of reactive oxygen species (ROS). Additionally, ssRNA40 promoted the expression of CD62L and TNF-α and the production of ROS in the presence of the TLR2 agonist Pam2CSK4. ssRNA40 together with R848 (an RIG-I/MDA5 agonist) increased CD11b expression but decreased CD62L expression. Furthermore, decreased IL-6 expression was observed in the presence of the TLR4 agonist LPS. Finally, we found that ssRNA40 promotes RIG-I and MDA5 expression in the presence of the TLR2, TLR4 and TLR7/8 agonists. This study demonstrates a functional response of TLRs in neutrophils challenged with ssRNA40, suggesting that TLRs could be involved in the innate immune response observed during HIV infection, which might be mediated by its genome.
Program: Medicina
Headquarters: Medellín
Publisher: Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Medicina, Medellín y Envigado, Colombia, 00000
Type: Artículo
CC Licence: Licencia CC
Citation: Giraldo D. M., Hernández, J. C.& Urcuqui Inchima, S. (2016). HIV-1-derived single-stranded RNA acts as activator of human neutrophils. Immunologic Research, 64 (5). 1185-1194. doi: 10.1007/s12026-016-8876-9
Resource reference: https://link.springer.com/article/10.1007%2Fs12026-016-8876-9
Appears in Collections:Medicina

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