Please use this identifier to cite or link to this item: http://repository.ucc.edu.co/handle/ucc/1186
Title: Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
Author: Peláez Vargas, Alejandro
Carvalho, Ángela
Hansford, Derek
Fernandes, María
Monteiro, Fernando J.
Email autor: alejandro.pelaezv@campusucc.edu.co
Issue Date: 2016
Keywords: Micropatrones;Biomateriales Dentales
Abstract: A primary goal in bone tissue engineering is the design of implants that induce controlled, guided, and rapid healing. The events that normally lead to the integration of an implant into bone and determine the performance of the device occur mainly at the tissue−implant interface. Topographical surface modification of a biomaterial might be an efficient tool for inducing stem cell osteogenic differentiation and replace the use of biochemical stimuli. The main goal of this work was to develop micropatterned bioactive silica thin films to induce the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) only through topographical stimuli. Line and pillar micropatterns were developed by a combination of sol−gel/soft lithography and characterized by scanning electron microscopy, atomic force microscopy, and contact angle measurements. hMSCs were cultured onto the microfabricated thin films and flat control for up to 21 days under basal conditions. The micropatterned groups induced levels of osteogenic differentiation and expression of osteoblast-associated markers higher than those of the flat controls. Via comparison of the micropatterns, the pillars caused a stronger response of the osteogenic differentiation of hMSCs with a higher level of expression of osteoblast-associated markers, ALP activity, and extracellular matrix mineralization after the cells had been cultured for 21 days. These findings suggest that specific microtopographic cues can direct hMSCs toward osteogenic differentiation.
Program: Odontología
Headquarters: Medellín
Publisher: Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Programa de Odontología, Medellín y Envigado, Colombia, 00000
Type: Artículo
CC Licence: Licencia CC
Citation: Peláez Vargas, A., Carvalho, A., Hansford, D., Fernandes, M, Monteiro, F. J. (2014). Effects of Line and Pillar Array Microengineered SiO2 Thin Films on the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells. Langmuir, 32, 1091-1100. doi: 10.1021/acs.langmuir.5b03955
Appears in Collections:Odontología

Files in This Item:
File Description SizeFormat 
Effects of line 1.pdf717.92 kBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.