Publicación: Efecto de protocolos de desinfección en pruebas microbiológicas y en la resistencia mecánica de los conos de gutapercha
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Introduction: Some studies have determined that gutta-percha cones are not 100% sterile, because they are contaminated during handling, storage and even from manufacturing, this leads to the use of contaminated cones during treatment, thus breaking the chain of asepsis and being able to generate treatment failure. Some studies have shown that gutta-percha cones are contaminated by facultative anaerobic bacteria, such as the genus Enterococcus faecalis and Bacillus spores, because these can not be disinfected in a conventional manner, it is necessary to establish an appropriate disinfection protocol, in addition to eliminate microorganisms that can be established in the root canal, will preserve the mechanical characteristics of these. Methodology: An experimental study was carried out to determine the effect of 5 disinfection protocols (2% glutaraldehyde for 1 minute, 6% Hydrogen Peroxide for 10 minutes, 6% Hydrogen Peroxide for 15 minutes, 2% Chlorhexidine for 1 minute and 2% chlorhexidine for 5 minutes) in gutta-percha cones, taking as a measure the ability to disinfect artificially contaminated cones with bacteria of Enterococus faecalis and spores of Geobacillus stearothermophilus, and the effect on the resistance of these materials. Results: it was detected that the disinfection with 2% Glutaraldehyde for 15 minutes and 6% hydrogen peroxide for 10 and 15 minutes, were effective to control the E. faecalis bacteria present in all artificially contaminated cones, while the protocols with 2% chlorhexidine for 1 and 5 minutes were effective to control the germination of G. stearothermophilus spores. In the mechanical part as results it was determined that the protocol that altered the resistance of the cone was the combination made between 6% Hydrogen Peroxide for 10 minutes + 2% Chlorhexidine for 1 minute. Conclusions: the effective treatments for E. faecallis are 2% glutaraldehyde for 15 minutes and 6% hydrogen peroxide for 10 and 15 minutes and for spores of G. stearothermophilus the chlorhexidine treatments were 2% for 1 and 5 minutes, and the one that altered the resistance of the cone was the combination made between 6% Hydrogen Peroxide for 10 minutes + 2% Chlorhexidine for 1 minute.